Transcriptome-wide Dynamics of m6A mRNA Methylation During Porcine Spermatogenesis

被引：20

作者：

Liu, Zidong ^{[1
]}

Chen, Xiaoxu ^{[1
]}

Zhang, Pengfei ^{[1
]}

Li, Fuyuan ^{[1
]}

Zhang, Lingkai ^{[1
]}

Li, Xueliang ^{[1
]}

Huang, Tao ^{[1
]}

Zheng, Yi ^{[1
]}

Yu, Taiyong ^{[1
]}

Zhang, Tao ^{[2
]}

Zeng, Wenxian ^{[1
]}

Lu, Hongzhao ^{[2
]}

Lv, Yinghua ^{[3
]}

机构：

[1] Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China

[2] Shaanxi Univ Technol, Sch Biol Sci & Engn, Hanzhong 723001, Peoples R China

[3] Northwest A&F Univ, Coll Chem & Pharm, Yangling 712100, Shaanxi, Peoples R China

来源：

GENOMICS PROTEOMICS & BIOINFORMATICS | 2023年 / 21卷 / 04期

基金：

中国国家自然科学基金;

关键词：

N-6-methyladenosine; Spermatogonial stem cell; Spermatogenesis; SETDB1; Pig; STEM-CELLS; SURVIVAL; TRANSLATION; MAINTENANCE;

D O I：

10.1016/j.gpb.2021.08.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Spermatogenesis is a continual process that occurs in the testes, in which diploid spermatogonial stem cells (SSCs) differentiate and generate haploid spermatozoa. This highly efficient and intricate process is orchestrated at multiple levels. N-6-methyladenosine (m(6)A), an epigenetic modification prevalent in mRNAs, is implicated in the transcriptional regulation during spermatogenesis. However, the dynamics of m(6)A modification in non-rodent mammalian species remains unclear. Here, we systematically investigated the profile and role of m(6)A during spermatogenesis in pigs. By analyzing the transcriptomic distribution of m(6)A in spermatogonia, spermatocytes, and round spermatids, we identified a globally conserved m(6)A pattern between porcine and murine genes with spermatogenic function. We found that m(6)A was enriched in a group of genes that specifically encode the metabolic enzymes and regulators. In addition, transcriptomes in porcine male germ cells could be subjected to the m(6)A modification. Our data show that m(6)A plays the regulatory roles during spermatogenesis in pigs, which is similar to that in mice. Illustrations of this point are three genes (SETDB1, FOXO1, and FOXO3) that are crucial to the determination of the fate of SSCs. To the best of our knowledge, this study for the first time uncovers the expression profile and role of m(6) Aduring spermatogenesis in large animals and provides insights into the intricate transcriptional regulation underlying the lifelong male fertility in non-rodent mammalian species.

引用

页码：729 / 741

页数：13

共 48 条

[1] The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice [J].

An, J. ;

Zhang, X. ;

Qin, J. ;

Wan, Y. ;

Hu, Y. ;

Liu, T. ;

Li, J. ;

Dong, W. ;

Du, E. ;

Pan, C. ;

Zeng, W. .

CELL DEATH & DISEASE, 2014, 5 :e1196-e1196

[2] DREME: motif discovery in transcription factor ChIP-seq data [J].

Bailey, Timothy L. .

BIOINFORMATICS, 2011, 27 (12) :1653-1659

[3] m6A RNA Modification Controls Cell Fate Transition in Mammalian Embryonic Stem Cells [J].

Batista, Pedro J. ;

Molinie, Benoit ;

Wang, Jinkai ;

Qu, Kun ;

Zhang, Jiajing ;

Li, Lingjie ;

Bouley, Donna M. ;

Lujan, Ernesto ;

Haddad, Bahareh ;

Daneshvar, Kaveh ;

Carter, Ava C. ;

Flynn, Ryan A. ;

Zhou, Chan ;

Lim, Kok-Seong ;

Dedon, Peter ;

Wernig, Marius ;

Mullen, Alan C. ;

Xing, Yi ;

Giallourakis, Cosmas C. ;

Chang, Howard Y. .

CELL STEM CELL, 2014, 15 (06) :707-719

[4] Transcription and post-transcriptional regulation of spermatogenesis [J].

Bettegowda, Anilkumar ;

Wilkinson, Miles F. .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2010, 365 (1546) :1637-1651

[5] Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor [J].

Brunet, A ;

Bonni, A ;

Zigmond, MJ ;

Lin, MZ ;

Juo, P ;

Hu, LS ;

Anderson, MJ ;

Arden, KC ;

Blenis, J ;

Greenberg, ME .

CELL, 1999, 96 (06) :857-868

[6] The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types [J].

Chen, Tingting ;

Chen, Xu ;

Zhang, Sisi ;

Zhu, Junwei ;

Tang, Bixia ;

Wang, Anke ;

Dong, Lili ;

Zhang, Zhewen ;

Yu, Caixia ;

Sun, Yanling ;

Chi, Lianjiang ;

Chen, Huanxin ;

Zhai, Shuang ;

Sun, Yubin ;

Lan, Li ;

Zhang, Xin ;

Xiao, Jingfa ;

Bao, Yiming ;

Wang, Yanqing ;

Zhang, Zhang ;

Zhao, Wenming .

GENOMICS PROTEOMICS & BIOINFORMATICS, 2021, 19 (04) :578-583

[7]

Chen XX, 2017, REPRODUCTION, V154, P789, DOI [10.1530/rep-17-0441, 10.1530/REP-17-0441]

[8] m6A RNA Methylation Regulates the Self-Renewal and Tumorigenesis of Glioblastoma Stem Cells [J].

Cui, Qi ;

Shi, Hailing ;

Ye, Peng ;

Li, Li ;

Qu, Qiuhao ;

Sun, Guoqiang ;

Sun, Guihua ;

Lu, Zhike ;

Huang, Yue ;

Yang, Cai-Guang ;

Riggs, Arthur D. ;

He, Chuan ;

Shi, Yanhong .

CELL REPORTS, 2017, 18 (11) :2622-2634

[9] IDENTIFICATION OF METHYLATED NUCLEOSIDES IN MESSENGER-RNA FROM NOVIKOFF HEPATOMA-CELLS [J].

DESROSIERS, R ;

FRIDERICI, K ;

ROTTMAN, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (10) :3971-3975

[10] African Swine Fever Epidemiology and Control [J].

Dixon, Linda K. ;

Stahl, Karl ;

Jori, Ferran ;

Vial, Laurence ;

Pfeiffer, Dirk U. .

ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 8, 2020, 2020, 8 :221-246

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