Production of monoclonal antibody and development of an icELISA for the detection of paxilline in grain

被引：2

作者：

Wang, Jiaxin ^{[1
]}

Shang, Wenhua ^{[1
]}

Shao, Shibei ^{[1
]}

Zhang, Jialin ^{[2
]}

Wu, Qiuyang ^{[1
]}

He, Min ^{[1
]}

Ma, Licai ^{[2
]}

Shen, Jianzhong ^{[1
]}

Wang, Zhanhui ^{[1
]}

Wen, Kai ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food S, Beijing Lab Food Qual & Safety, Beijing, Peoples R China

[2] Beijing WDWK Biotechnol Co Ltd, Res & Dev Dept, Beijing, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2024年 / 35卷 / 01期

关键词：

Paxilline; monoclonal antibody; icELISA; immunoassay; MYCOTOXIN PAXILLINE; ERGOT; IMMUNOASSAYS; PERAMINE; LOLII; FUNGI; ASSAY;

D O I：

10.1080/09540105.2023.2255759

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Paxilline (PAX) is a tremorgenic mycotoxin that is thought to be associated with various syndromes in animals. To facilitate the determination of this toxin, a hapten was chemically synthesized and then respectively conjugated to Bovine Thyroglobulin (BTG) and Bovine serum albumin (BSA) for antibody production. Monoclonal antibody (mAb) 4F8 was finally obtained and used to establish an accurate and sensitive indirect competitive ELISA (icELISA). Various parameters that could impact the performance of icELISA were optimized, including the ion concentration, pH value and organic solvent. Under optimal condition, this assay exhibits an IC50 value as low as 0.84 mu g L-1. The limit of detection (LOD) of the established icELISA method for PAX was 0.03-0.3 mu g L-1. In conclusion, this study provided a rapid, sensitive, and high-throughput immunoassay for the detection of PAX in grains.

引用

页数：13

共 22 条

[11] Mycotoxins in horse feed
Liesener K.
Curtui V.
Dietrich R.
Märtlbauer E.
Usleber E.
[J]. Mycotoxin Research, 2010, 26 (1) : 23 - 30
[12] Development and Evaluation of Monoclonal Antibodies for Paxilline
Maragos, Chris M.
[J]. TOXINS, 2015, 7 (10): : 3903 - 3915
[13] Fluxapyroxad Haptens and Antibodies for Highly Sensitive Immunoanalysis of Food Samples
Mercader, Josep V.
Abad-Somovilla, Antonio
Agullo, Consuelo
Abad-Fuentes, Antonio
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65 (42) : 9333 - 9341
[14] Miles CO, 1998, APPL ENVIRON MICROB, V64, P601
[15] Evolutionary history of ergot with a new infrageneric classification (Hypocreales: Clavicipitaceae: Claviceps)
Pichova, Kamila
Pazoutova, Sylvie
Kostovcik, Martin
Chudickova, Milada
Stodulkova, Eva
Novak, Petr
Flieger, Miroslav
van der Linde, Elna
Kolarik, Miroslav
[J]. MOLECULAR PHYLOGENETICS AND EVOLUTION, 2018, 123 : 73 - 87
[16] LOLITREMS, PERAMINE AND PAXILLINE - MYCOTOXINS OF THE RYEGRASS ENDOPHYTE INTERACTION
ROWAN, DD
[J]. AGRICULTURE ECOSYSTEMS & ENVIRONMENT, 1993, 44 (1-4) : 103 - 122
[17] Synthesis and characterization of hapten-protein conjugates for antibody production against small molecules
Singh, KV
Kaur, J
Varshney, GC
Raje, M
Suri, CR
[J]. BIOCONJUGATE CHEMISTRY, 2004, 15 (01) : 168 - 173
[18] Hapten synthesis, monoclonal antibody production and development of a competitive indirect enzyme-linked immunosorbent assay for erythromycin in milk
Wang, Zhanhui
Mi, Tiejun
Beier, Ross C.
Zhang, Huiyan
Sheng, Yajie
Shi, Weimin
Zhang, Suxia
Shen, Jianzhong
[J]. FOOD CHEMISTRY, 2015, 171 : 98 - 107
[19] Development of sensitive and fast immunoassays for amantadine detection
Xu, Liguang
Peng, Shuang
Liu, Liqiang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
[J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2016, 27 (05) : 678 - 688
[20] Fluorescence Polarization Immunoassay Based on a New Monoclonal Antibody for the Detection of the Zearalenone Class of Mycotoxins in Maize
Zhang, Xiya
Eremin, Sergei A.
Wen, Kai
Yu, Xuezhi
Li, Chenglong
Ke, Yuebin
Jiang, Haiyang
Shen, Jianzhong
Wang, Zhanhui
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65 (10) : 2240 - 2247

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