Production of monoclonal antibody and development of an icELISA for the detection of paxilline in grain

被引：2

作者：

Wang, Jiaxin ^{[1
]}

Shang, Wenhua ^{[1
]}

Shao, Shibei ^{[1
]}

Zhang, Jialin ^{[2
]}

Wu, Qiuyang ^{[1
]}

He, Min ^{[1
]}

Ma, Licai ^{[2
]}

Shen, Jianzhong ^{[1
]}

Wang, Zhanhui ^{[1
]}

Wen, Kai ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food S, Beijing Lab Food Qual & Safety, Beijing, Peoples R China

[2] Beijing WDWK Biotechnol Co Ltd, Res & Dev Dept, Beijing, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2024年 / 35卷 / 01期

关键词：

Paxilline; monoclonal antibody; icELISA; immunoassay; MYCOTOXIN PAXILLINE; ERGOT; IMMUNOASSAYS; PERAMINE; LOLII; FUNGI; ASSAY;

D O I：

10.1080/09540105.2023.2255759

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Paxilline (PAX) is a tremorgenic mycotoxin that is thought to be associated with various syndromes in animals. To facilitate the determination of this toxin, a hapten was chemically synthesized and then respectively conjugated to Bovine Thyroglobulin (BTG) and Bovine serum albumin (BSA) for antibody production. Monoclonal antibody (mAb) 4F8 was finally obtained and used to establish an accurate and sensitive indirect competitive ELISA (icELISA). Various parameters that could impact the performance of icELISA were optimized, including the ion concentration, pH value and organic solvent. Under optimal condition, this assay exhibits an IC50 value as low as 0.84 mu g L-1. The limit of detection (LOD) of the established icELISA method for PAX was 0.03-0.3 mu g L-1. In conclusion, this study provided a rapid, sensitive, and high-throughput immunoassay for the detection of PAX in grains.

引用

页数：13

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