Mass spectrometry-based untargeted metabolomics study of non-obese individuals with non-alcoholic fatty liver disease

被引:9
作者
Demirel, Metin [1 ,2 ,7 ]
Koktasoglu, Fatmanur [1 ]
Ozkan, Esin [3 ]
Agac, Halime Dulun [1 ]
Gul, Ayse Zehra [1 ]
Sharifov, Rasul [4 ]
Sarikaya, Ufuk [2 ,5 ]
Basaranoglu, Metin [6 ]
Selek, Sahabettin [1 ]
机构
[1] Bezmialem Vakif Univ, Dept Med Biochem, Fac Med, Istanbul, Turkiye
[2] Bezmialem Vakif Univ, Hlth Sci Inst, Istanbul, Turkiye
[3] Bezmialem Vakif Univ, Fac Med, Istanbul, Turkiye
[4] Bezmialem Vakif Univ, Dept Radiol, Dept Gastroenterol, Istanbul, Turkiye
[5] Bezmialem Vakif Univ, Vocat Sch Hlth Serv, Dept Med Serv & Tech, Istanbul, Turkiye
[6] Bezmialem Vakif Univ, Dept Gastroenterol & Hepatol, Istanbul, Turkiye
[7] Bezmialem Vakif Univ, Dept Med Biochem, TR-34093 Istanbul, Turkiye
关键词
Metabolomics; non-obese; non-alcoholic fatty liver disease; nafld; metabolites; HISTAMINE; MODELS;
D O I
10.1080/00365521.2023.2225667
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
ObjectivesNon-alcoholic fatty liver disease (NAFLD) is a disease characterized by the accumulation of excessive fat in the liver, which can lead to fibrosis and has an increasing prevalence. NAFLD requires non-invasive diagnostic biomarkers. While typically observed in overweight individuals, it can also occur in non-obese/non-overweight individuals. Comparative studies on non-obese NAFLD patients are scarce. This study aimed to conduct a using liquid chromatography-high resolution mass spectrometry (LC-MS/MS)-based metabolic profiling of non-obese NAFLD patients and healthy controls.Materials and methodsThe patient group consisted of 27 individuals with NAFLD, while the healthy control group included 39 individuals. Both groups were between 18 and 40 years old, had a BMI of less than 25 and had alcohol consumption less than 20 g/week for men and 10 g/week for women. Serum samples were collected and analyzed using LC-MS/MS. The data were analyzed using the TidyMass and MetaboAnalyst.ResultsThe LC-MS/MS analyses detected significant changes in D-amino acid metabolism, vitamin B6 metabolism, apoptosis, mTOR signaling pathway, lysine degradation, and phenylalanine metabolism pathways in non-obese NAFLD patients. Significant changes were also observed in the metabolites D-pantothenic acid, hypoxanthine, citric acid, citramalic acid, L-phenylalanine, glutamine, and histamine-trifluoromethyl-toluidide, & beta;-hydroxymyristic acid, DL-Lactic acid, and 3-methyl-2-oxopentanoic. Overall, the study provides valuable insights into the metabolic changes associated with non-obese NAFLD patients and can contribute to the development of non-invasive diagnostic biomarkers for NAFLD.ConclusionsThis study sheds light on the metabolic changes in non-obese NAFLD patients. Further research is needed to better understand the metabolic changes associated with NAFLD and to develop effective treatment options.
引用
收藏
页码:1344 / 1350
页数:7
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