PD-1highCXCR5-CD4+peripheral helper T cells promote CXCR3+plasmablasts in human acute viral infection

被引：19

作者：

Asashima, Hiromitsu ^{[1
,2
]}

Mohanty, Subhasis ^{[3
]}

Comi, Michela ^{[1
,2
]}

Ruff, William E. ^{[1
,2
]}

Hoehn, Kenneth B. ^{[1
]}

Wong, Patrick ^{[2
]}

Klein, Jon ^{[2
]}

Lucas, Carolina ^{[2
]}

Cohen, Inessa ^{[1
,2
]}

Coffey, Sarah ^{[1
,2
]}

Lele, Nikhil ^{[1
,2
]}

Greta, Leissa ^{[1
,2
]}

Raddassi, Khadir ^{[1
,2
]}

Chaudhary, Omkar ^{[3
]}

Unterman, Avraham ^{[5
]}

Emu, Brinda ^{[3
]}

Kleinstein, Steven H. ^{[2
,4
,6
]}

Montgomery, Ruth R. ^{[7
]}

Iwasaki, Akiko ^{[2
,3
,8
]}

Cruz, Charles S. Dela ^{[5
]}

Kaminski, Naftali ^{[5
]}

Shaw, Albert C. ^{[3
]}

Hafler, David A. ^{[1
,2
]}

Sumida, Tomokazu S. ^{[1
]}

机构：

[1] Yale Sch Med, Dept Neurol, New Haven, CT 06510 USA

[2] Yale Sch Med, Dept Immunobiol, New Haven, CT USA

[3] Yale Univ, Yale Sch Med, Dept Internal Med, Sect Infect Dis, New Haven, CT USA

[4] Yale Sch Med, Dept Pathol, New Haven, CT USA

[5] Yale Univ, Sch Med, Dept Internal Med, Sect Pulm,Crit Care & Sleep Med Sect, New Haven, CT USA

[6] Yale Univ, Program Computat Biol & Bioinformat, New Haven, CT USA

[7] Yale Sch Med, Dept Internal Med, New Haven, CT USA

[8] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

CELL REPORTS | 2023年 / 42卷 / 01期

基金：

美国国家卫生研究院;

关键词：

MEMORY B-CELLS; GERMINAL-CENTERS; VIRUS-INFECTION; IMMUNOGLOBULIN; DIFFERENTIATION; GENERATION; RESPONSES;

D O I：

10.1016/j.celrep.2022.111895

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

T cell-B cell interaction is the key immune response to protect the host from severe viral infection. However, how T cells support B cells to exert protective humoral immunity in humans is not well understood. Here, we use COVID-19 as a model of acute viral infections and analyze CD4+ T cell subsets associated with plasma -blast expansion and clinical outcome. Peripheral helper T cells (Tph cells; denoted as PD-1highCXCR5-CD4+ T cells) are significantly increased, as are plasmablasts. Tph cells exhibit "B cell help"signatures and induce plasmablast differentiation in vitro. Interestingly, expanded plasmablasts show increased CXCR3 expres-sion, which is positively correlated with higher frequency of activated Tph cells and better clinical outcome. Mechanistically, Tph cells help B cell differentiation and produce more interferon g (IFNg), which induces CXCR3 expression on plasmablasts. These results elucidate a role for Tph cells in regulating protective B cell response during acute viral infection.

引用

页数：19

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