Development of highly soluble and functional buffalo milk protein concentrate 60 by modifying ionic environment and characterisation thereof

The ionic and protein environment of buffalo skim milk was modified by instant pH drop and its restoration for the development of highly soluble milk protein concentrate-60 (BuMRate-60(@)), which resulted in depletion of 88.64% calcium, 89.18% magnesium, 91.69% potassium and 93.96% phosphorous. BuMRate-60(@) displayed excellent wetting, rehydration and solubility (97.76%). The Fourier transform infrared spectrometer spectra revealed significant stretching (C-H and O-H), N-H bending and a large extent of H-bonding. Scanning electron microscopy micrographs presented particles with porous nature and dimples, while transmission electron microscopy confirmed a greater release of minerals with altered casein micelles.