High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation

被引：23

作者：

Laine, Romain F. ^{[1
,2
,16
]}

Heil, Hannah S. ^{[3
]}

Coelho, Simao ^{[3
]}

Nixon-Abell, Jonathon ^{[4
,5
]}

Jimenez, Angelique ^{[6
]}

Wiesner, Theresa ^{[6
]}

Martinez, Damian ^{[3
]}

Galgani, Tommaso ^{[7
,17
]}

Regnier, Louise ^{[7
]}

Stubb, Aki ^{[8
,9
,18
]}

Follain, Gautier ^{[8
,9
,10
]}

Webster, Samantha ^{[11
]}

Goyette, Jesse ^{[11
]}

Dauphin, Aurelien ^{[12
]}

Salles, Audrey ^{[13
]}

Culley, Sian ^{[1
,19
]}

Jacquemet, Guillaume ^{[8
,9
,10
,14
,15
]}

Hajj, Bassam ^{[7
]}

Leterrier, Christophe ^{[6
]}

Henriques, Ricardo ^{[1
,2
,3
]}

机构：

[1] UCL, Lab Mol Cell Biol, London, England

[2] Francis Crick Inst, London, England

[3] Inst Gulbenkian Ciencias, Opt Cell Biol, Oeiras, Portugal

[4] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA

[5] Cambridge Inst Med Res, Cambridge Univeristy, Cambridge, England

[6] Aix Marseille Univ, CNRS, INP UMR7051, NeuroCyto, Marseille, France

[7] Sorbonne Univ, PSL Res Univ, Inst Curie, Lab Physico Chim Curie,CNRS UMR168, Paris, France

[8] Univ Turku, Turku Biosci Ctr, Turku, Finland

[9] Abo Akad Univ, Turku, Finland

[10] Abo Akad Univ, Fac Sci & Engn, Cell Biol, Turku, Finland

[11] Univ New South Wales, Sch Biomed Sci, EMBL Australia Node Single Mol Sci, Sydney, NSW, Australia

[12] PSL Res Univ, Inst Curie, CNRS, INSERM,Unite Genet & Biol Dev U934,PCT,IBiSA, F-75248 Paris, France

[13] Univ Paris Cite, Inst Pasteur, C2RT, Unit Technol & Serv Photon BioImaging UTechS PBI, Paris, France

[14] Univ Turku, Turku Bioimaging, Turku, Finland

[15] Abo Akad Univ, InFLAMES Res Flagship Ctr, Turku, Finland

[16] Micrograph Bio, Translat & Innovat Hub, London, England

[17] Revv Signals, Tres Cantos, Madrid, Spain

[18] Max Planck Inst Mol Biomed, Dept Cell & Tissue Dynam, Munster, Germany

[19] Kings Coll London, Randall Ctr Cell & Mol Biophys, Guys Campus, London, England

来源：

NATURE METHODS | 2023年 / 20卷 / 12期

基金：

欧盟地平线“2020”; 芬兰科学院; 英国惠康基金; 欧洲研究理事会; 英国医学研究理事会;

关键词：

QUALITY ASSESSMENT; RESOLUTION LIMIT; MICROSCOPY; LOCALIZATION; RECONSTRUCTION; MOLECULES; BINDING;

D O I：

10.1038/s41592-023-02057-w

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below the diffraction limit. Here we present enhanced super-resolution radial fluctuations (eSRRF), substantially improving image fidelity and resolution compared to the original SRRF method. eSRRF incorporates automated parameter optimization based on the data itself, giving insight into the trade-off between resolution and fidelity. We demonstrate eSRRF across a range of imaging modalities and biological systems. Notably, we extend eSRRF to three dimensions by combining it with multifocus microscopy. This realizes live-cell volumetric super-resolution imaging with an acquisition speed of similar to 1 volume per second. eSRRF provides an accessible super-resolution approach, maximizing information extraction across varied experimental conditions while minimizing artifacts. Its optimal parameter prediction strategy is generalizable, moving toward unbiased and optimized analyses in super-resolution microscopy.

引用

页码：1949 / 1956

页数：25

共 4 条

[1] Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
Gustafsson, Nils
Culley, Sian
Ashdown, George
Owen, Dylan M.
Pereira, Pedro Matos
Henriques, Ricardo
NATURE COMMUNICATIONS, 2016, 7
[2] Live-cell 3D super-resolution imaging in thick biological samples
Zanacchi, Francesca Cella
Lavagnino, Zeno
Donnorso, Michela Perrone
Del Bue, Alessio
Furia, Laura
Faretta, Mario
Diaspro, Alberto
NATURE METHODS, 2011, 8 (12) : 1047 - +
[3] 3D Bayesian cluster analysis of super-resolution data reveals LAT recruitment to the T cell synapse
Griffie, Juliette
Shlomovich, Leigh
Williamson, David J.
Shannon, Michael
Aaron, Jesse
Khuon, Satya
Burn, Garth L.
Boelen, Lies
Peters, Ruby
Cope, Andrew P.
Cohen, Edward A. K.
Rubin-Delanchy, Patrick
Owen, Dylan M.
SCIENTIFIC REPORTS, 2017, 7
[4] Super-Resolution Two-Photon Fluorescence Tomography Through the Phase-Shifted Optical Beatings of Bessel Beams for High-Resolution Deeper Tissue 3D Imaging
Lin, Shulang
Gong, Li
Huang, Zhiwei
LASER & PHOTONICS REVIEWS, 2024, 18 (02)

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