Analysis of an IncR plasmid carried by carbapenem-resistant Klebsiella pneumoniae: A survey of swine Klebsiella pneumoniae in Jilin Province

被引：7
作者：
Qi, Yu [1 ]
Xue, Jun-Ze [1 ]
Li, Shuang-shuang [1 ]
Elken, Emad Mohammed [1 ,4 ]
Haqmal, M. Aman [1 ]
Li, Xue-Song [1 ]
Xu, Guan-Yi [1 ]
Kong, Ling-Cong [1 ,2 ,5 ]
Ma, Hong-Xia [1 ,2 ,3 ,5 ]
机构：
[1] Jilin Agr Univ, Coll Anim Sci & Technol, Dept Vet Med, Changchun, Peoples R China
[2] Jilin Agr Univ, Key Lab New Vet Drug Res & Dev Jilin Prov, Changchun, Peoples R China
[3] Jilin Agr Univ, Engn Res Ctr Bioreactor & Drug Dev, Minist Educ, Changchun, Peoples R China
[4] Al Azhar Univ, Fac Agr, Dept Anim Prod, Cairo, Egypt
[5] Jilin Agr Univ, Coll Anim Sci & Technol, Dept Vet Med, Xincheng St 2888, Changchun 130118, Peoples R China
来源：
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE | 2023年 / 34卷
基金：
中国国家自然科学基金;
关键词：
Antibiotic resistance; Antibiotic resistance genes; Genome sequencing; IncR plasmid; Klebsiella pneumoniae; ESCHERICHIA-COLI; ANTIBIOTIC-RESISTANCE; GENES;
D O I：
10.1016/j.jgar.2023.04.018
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Objectives: This study was conducted in Jilin Province to investigate the mechanism involved in the an-tibiotic resistance and pathogenicity of Klebsiella pneumoniae. Methods: Lung samples were collected from large-scale pig farms in Jilin Province. Antimicrobial suscep-tibility and mouse lethality assays were carried out. K. pneumoniae isolate JP20, with high virulence and antibiotic resistance, was chosen for whole-genome sequencing. The complete sequence of its genome was annotated, and the virulence and antibiotic resistance mechanism were analysed. Results: A total of 32 K. pneumoniae strains were isolated and tested for antibiotic resistance and pathogenicity. Among them, the JP20 strain showed high levels of resistance to all tested antimicro-bial agents and strong pathogenicity in mice (lethal dose of 1.35 x 10 11 CFU/mL). Sequencing of the multidrug-resistant and highly virulent K. pneumoniae JP20 strain revealed that the antibiotic resistance genes were mainly carried by an IncR plasmid. We speculate that extended-spectrum & beta;-lactamases and loss of outer membrane porin OmpK36 play an important role in carbapenem antibiotic resistance. This plasmid contains a mosaic structure consisting of a large number of mobile elements. Conclusion: Through genome-wide analysis, we found that an lncR plasmid carried by the JP20 strain may have evolved in pig farms, possibly leading to multidrug resistance in the JP20 strain. It is speculated that the antibiotic resistance of K. pneumoniae in pig farms is mainly mediated by mobile elements (insertion sequences, transposons, and plasmids). These data provide a basis for monitoring the antibiotic resistance of K. pneumoniae and lay a foundation for an improved understanding of the genomic characteristics and antibiotic resistance mechanism of K. pneumoniae. & COPY; 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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页码：83 / 90
页数：8
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