Genome-wide identification of candidate chemosensory receptors in the bean bug Riptortus pedestris (Hemiptera: Alydidae) and the functional verification of its odorant receptor co-receptor (Orco) in recognizing aggregation pheromone

被引：4

作者：

Liu, Panjing ^{[1
]}

Guo, Jianglong ^{[1
]}

Wei, Hongyi ^{[2
]}

Feng, Likai ^{[3
]}

Gao, Zhanlin ^{[1
]}

Zhang, Tao ^{[1
]}

机构：

[1] Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Integrated Pest Management Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China,Minist, Baoding, Peoples R China

[2] Jiangxi Agr Univ, Inst Entomol, Nanchang, Peoples R China

[3] Xinjiang Acad Agr & Reclamat Sci, Inst Plant Protect, Shihezi, Peoples R China

来源：

FRONTIERS IN PHYSIOLOGY | 2023年 / 14卷

关键词：

the bean bug; olfactory recognition; genome-wide; pheromone; RNAi; RIPTORTUS-PEDESTRIS FABRICIUS; OLFACTORY RECEPTORS; GUSTATORY RECEPTORS; GENE; EXPRESSION; EVOLUTION; PROTEINS; HETEROPTERA; INSIGHTS; BEHAVIOR;

D O I：

10.3389/fphys.2023.1224009

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

A sophisticated and sensitive olfactory system plays a vital role in the survival and reproduction of insects. Chemosensory receptors are indispensable for the molecular recognition and discrimination of semiochemicals. Riptortus pedestris is a notorious pest of legume plants, resulting in yield losses and quality decreases in soybeans. It is well accepted that R. pedestris highly relies on its olfactory system in detecting aggregation pheromones, host volatiles, and pesticides; however, little research focused on its chemosensory receptors. In the present study, we identified 237 odorant receptors (ORs), 42 gustatory receptors (GRs), and 31 ionotropic receptors (IRs) from the reported genome of R. pedestris, and analyzed their phylogenetic relationship with other hemipteran species. Through the results of RNA-seq and real-time quantitative PCR (qRT-PCR), we found that RpedORs displayed different expression levels in the antennae of R. pedestris at different development stages. To further verify the function of odorant receptor co-receptor (Orco), an obligate and unique insect OR, we silenced RpedOrco by RNA interference (RNAi) method. The results showed that silencing RpedOrco could significantly impair the response to aggregation pheromone in R. pedestris, indicating that RpedOrco plays an essential role in odorant detection. Our results can provide the theoretical foundations for revealing the olfactory recognition mechanism of R. pedestris and help explore and develop novel olfactory-based agents against this pest.

引用

页数：14