Conventional retention models lead to accurate descriptions of the elution behaviour from the fitting of data for single solutes or from a set of solutes, one by one. However, the simultaneous fitting of several solutes through a regression process that separates the contributions of column and solvent from those of each solute is also possible. The result is a global retention model constituted by a set of equations with some common parameters (those associated with column and solvent), whereas others, specific to each solute, differ for each equation. This work explores the possibilities, advantages, and limitations of global models when they are applied to the optimisation of chromatographic resolution. A set constituted by 13 drugs (diuretics and beta-blockers) and a training experimental design of seven multi-linear gradients are considered. Since standards for all compounds were available, the optimisation based on global models could be compared with the conventional optimisation, which is based on individual models. In their current state, global models do not predict changes in elution order, but they do allow for incorporating additional solutes (e.g., new analytes or matrix peaks) with only one new experiment. This possibility is explored by extending the model for the 13 analytes to include 26 peaks associated with a contamination in the injector. The combination of individual and global models allows an optimisation where the effects of matrix peaks on the separation of analytes can be integrated.(c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
