Protocol Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

被引：2

作者：

Song, Jinyuan ^{[1
,2
,3
]}

Zhang, Junlei ^{[1
,2
,3
]}

Ji, Yongtao ^{[1
,2
,3
]}

Tang, Jianghui ^{[1
,2
,3
]}

Sheng, Jianpeng ^{[1
,2
,3
]}

Liang, Tingbo ^{[1
,2
,3
]}

Bai, Xueli ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, Hangzhou 310002, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Zhejiang Prov Key Lab Pancreat Dis, Hangzhou 310002, Peoples R China

[3] Zhejiang Univ, Canc Ctr, Hangzhou 310002, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Cancer; Cell Biology; Cell Isolation; Clinical Protocol; Flow Cytometry; Health Sciences; Single Cell;

D O I：

10.1016/j.xpro.2023.102679

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1

引用

页数：11