Augmenting the Angiogenic Profile and Functionality of Cord Blood Endothelial Colony-Forming Cells by Indirect Priming with Bone-Marrow-Derived Mesenchymal Stromal Cells

被引:0
作者
Bansal, Ashutosh [1 ]
Singh, Archna [2 ]
Nag, Tapas Chandra [3 ]
Sharma, Devyani [3 ]
Garg, Bhavuk [4 ]
Bhatla, Neerja [5 ]
Choudhury, Saumitra Dey [6 ]
Ramakrishnan, Lakshmy [1 ]
机构
[1] All India Inst Med Sci, Dept Cardiac Biochem, New Delhi 110029, India
[2] All India Inst Med Sci, Dept Biochem, New Delhi 110029, India
[3] All India Inst Med Sci, Dept Anat, New Delhi 110029, India
[4] All India Inst Med Sci, Dept Orthopaed, New Delhi 110029, India
[5] All India Inst Med Sci, Dept Obstet & Gynaecol, New Delhi 110029, India
[6] All India Inst Med Sci, Centralized Core Res Facil, New Delhi 110029, India
关键词
endothelial colony-forming cells (ECFCs); bone-marrow-derived mesenchymal stem/ stromal cells (BM-MSCs); co-culture; direct priming; indirect priming; functionality of ECFCs; proteome profiling; PROGENITOR CELLS; GROWTH; PROLIFERATION;
D O I
10.3390/biomedicines11051372
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cellular therapy has shown promise as a strategy for the functional restoration of ischemic tissues through promoting vasculogenesis. Therapy with endothelial progenitor cells (EPCs) has shown encouraging results in preclinical studies, but the limited engraftment, inefficient migration, and poor survival of patrolling endothelial progenitor cells at the injured site hinder its clinical utilization. These limitations can, to some extent, be overcome by co-culturing EPCs with mesenchymal stem cells (MSCs). Studies on the improvement in functional capacity of late EPCs, also referred to as endothelial colony-forming cells (ECFCs), when cultured with MSCs have mostly focused on the angiogenic potential, although migration, adhesion, and proliferation potential also determine effective physiological vasculogenesis. Alteration in angiogenic proteins with co-culturing has also not been studied. We co-cultured ECFCs with MSCs via both direct and indirect means, and studied the impact of the resultant contact-mediated and paracrine-mediated impact of MSCs over ECFCs, respectively, on the functional aspects and the angiogenic protein signature of ECFCs. Both directly and indirectly primed ECFCs significantly restored the adhesion and vasculogenic potential of impaired ECFCs, whereas indirectly primed ECFCs showed better proliferation and migratory potential than directly primed ECFCs. Additionally, indirectly primed ECFCs, in their angiogenesis proteomic signature, showed alleviated inflammation, along with the balanced expression of various growth factors and regulators of angiogenesis.
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页数:20
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