Liquid chromatography-mass spectrometry based metabolomics investigation of different tissues of Mytilus galloprovincialis

被引:3
|
作者
James, Lea [1 ]
Gomez, Elena [1 ]
Ramirez, Gaelle [1 ]
Dumas, Thibaut [1 ]
Courant, Frederique [1 ,2 ]
机构
[1] Univ Montpellier, CNRS, IRD, Hydrosci Montpellier, Montpellier, France
[2] Hydrosci Montpellier, 15 Ave Charles Flahault, F-34090 Montpellier, France
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2023年 / 45卷
关键词
Metabolomics; Bivalve; Gonad; Foot; Digestive gland; Gill; Soft tissue; MUSSEL PERNA-VIRIDIS; CYCLES; METABOLITES; RESPONSES; EXPOSURE;
D O I
10.1016/j.cbd.2022.101051
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Mediterranean mussel (Mytilus galloprovincialis) is widely used in monitoring programs and in ecotoxico-logical studies to examine the biological effects of physicochemical parameter changes and the impact of chemical pollutants. Metabolomics has recently demonstrated high potential to gain further insight into the molecular effects of chemical exposure and the success of its application is dependent on the extent of prior metabolomics knowledge available on the target organism. Therefore, the purpose of this study was the inves-tigation of the metabolites of five different functional tissues of male and female Mediterranean mussels (digestive gland, foot, gill and gonad tissues and in the remaining soft tissues) accessible to the analysis using the most common sample preparation recommended for tissue analysis (i.e. Bligh & Dyer). Metabolic fingerprints were acquired via liquid chromatography high-resolution mass spectrometry and the identification was based on an internal database developed in the laboratory. It led to the identification of 110 metabolites, among which amino acids, carboxylic acids, purine and pyrimidine metabolites were often the most abundant. The metabolic contents of the five tissues quantitatively and qualitatively differed, with a clear distinction between male and female contents observed in the gonads and digestive glands. These results underline the importance of selecting the most suitable tissue and sex to study the impact of contamination on metabolism and the need for further research to deeper characterize the metabolome of this organism.
引用
收藏
页数:8
相关论文
共 50 条
  • [41] Feasibility of Detecting Prostate Cancer by Ultraperformance Liquid Chromatography-Mass Spectrometry Serum Metabolomics
    Zang, Xiaoling
    Jones, Christina M.
    Long, Tran Q.
    Monge, Maria Eugenia
    Zhou, Manshui
    Walker, L. DeEtte
    Mezencev, Roman
    Gray, Alexander
    McDonald, John F.
    Fernandez, Facundo M.
    JOURNAL OF PROTEOME RESEARCH, 2014, 13 (07) : 3444 - 3454
  • [42] Advanced liquid chromatography-mass spectrometry enables merging widely targeted metabolomics and proteomics
    Liu, Wenjing
    Song, Qingqing
    Cao, Yan
    Zhao, Yanan
    Huo, Huixia
    Wang, Yitao
    Song, Yuelin
    Li, Jun
    Tu, Pengfei
    ANALYTICA CHIMICA ACTA, 2019, 1069 : 89 - 97
  • [43] Endocannabinoid metabolomics: A novel liquid chromatography-mass spectrometry reagent for fatty acid analysis
    Williams, John
    Pandarinathan, Lakshmipathi
    Wood, JodiAnne
    Vouros, Paul
    Makriyannis, Alexandros
    AAPS JOURNAL, 2006, 8 (04): : E655 - E660
  • [44] Formation of sodium cluster ions complicates liquid chromatography-mass spectrometry metabolomics analyses
    Carnevale Neto, Fausto
    Pascua, Vadim
    Raftery, Daniel
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2021, 35 (20)
  • [45] Application of liquid chromatography-mass spectrometry to the investigation of poisoning by Oenanthe crocata
    Kite, Geoffrey C.
    Stoneham, Charlotte A.
    Veitch, Nigel C.
    Stein, Bridget K.
    Whitwell, Katherine E.
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 838 (01): : 63 - 70
  • [46] Determination of chloramphenicol in animal tissues and urine - Liquid chromatography-tandem mass spectrometry versus gas chromatography-mass spectrometry
    Gantverg, A
    Shishani, I
    Hoffman, M
    ANALYTICA CHIMICA ACTA, 2003, 483 (1-2) : 125 - 135
  • [47] Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics
    Xie, Fang
    Liu, Tao
    Qian, Wei-Jun
    Petyuk, Vladislav A.
    Smith, Richard D.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (29) : 25443 - 25449
  • [48] LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY-BASED PROTEOMICS OF NITROSOMONAS
    Wessels, Hans J. C. T.
    Gloerich, Jolein
    van der Biezen, Erwin
    Jetten, Mike S. M.
    Kartal, Boran
    METHODS IN ENZYMOLOGY: RESEARCH ON NITRIFICATION AND RELATED PROCESSES, VOL 486, PART A, 2011, 486 : 465 - 482
  • [49] Non-targeted metabolomics study for the analysis of chemical compositions in three types of tea by using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry
    Zhang Lei
    Zeng Zhongda
    Ye Guozhu
    Zhao Chunxia
    Lu Xin
    Xu Guowang
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2014, 32 (08) : 804 - 816
  • [50] Toward Accurate and Robust Liquid Chromatography-Mass Spectrometry-Based Quantification of Antibody Biotherapeutics in Tissues
    An, Bo
    Zhang, Ming
    Pu, Jie
    Qu, Yang
    Shen, Shichen
    Zhou, Shaolian
    Ferrari, Luca
    Vazvaei, Faye
    Qu, Jun
    ANALYTICAL CHEMISTRY, 2020, 92 (22) : 15152 - 15161