Liquid chromatography-mass spectrometry based metabolomics investigation of different tissues of Mytilus galloprovincialis

被引:3
|
作者
James, Lea [1 ]
Gomez, Elena [1 ]
Ramirez, Gaelle [1 ]
Dumas, Thibaut [1 ]
Courant, Frederique [1 ,2 ]
机构
[1] Univ Montpellier, CNRS, IRD, Hydrosci Montpellier, Montpellier, France
[2] Hydrosci Montpellier, 15 Ave Charles Flahault, F-34090 Montpellier, France
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2023年 / 45卷
关键词
Metabolomics; Bivalve; Gonad; Foot; Digestive gland; Gill; Soft tissue; MUSSEL PERNA-VIRIDIS; CYCLES; METABOLITES; RESPONSES; EXPOSURE;
D O I
10.1016/j.cbd.2022.101051
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Mediterranean mussel (Mytilus galloprovincialis) is widely used in monitoring programs and in ecotoxico-logical studies to examine the biological effects of physicochemical parameter changes and the impact of chemical pollutants. Metabolomics has recently demonstrated high potential to gain further insight into the molecular effects of chemical exposure and the success of its application is dependent on the extent of prior metabolomics knowledge available on the target organism. Therefore, the purpose of this study was the inves-tigation of the metabolites of five different functional tissues of male and female Mediterranean mussels (digestive gland, foot, gill and gonad tissues and in the remaining soft tissues) accessible to the analysis using the most common sample preparation recommended for tissue analysis (i.e. Bligh & Dyer). Metabolic fingerprints were acquired via liquid chromatography high-resolution mass spectrometry and the identification was based on an internal database developed in the laboratory. It led to the identification of 110 metabolites, among which amino acids, carboxylic acids, purine and pyrimidine metabolites were often the most abundant. The metabolic contents of the five tissues quantitatively and qualitatively differed, with a clear distinction between male and female contents observed in the gonads and digestive glands. These results underline the importance of selecting the most suitable tissue and sex to study the impact of contamination on metabolism and the need for further research to deeper characterize the metabolome of this organism.
引用
收藏
页数:8
相关论文
共 50 条
  • [31] A Review of Liquid Chromatography-Mass Spectrometry Strategies for the Analyses of Metabolomics Induced by Microplastics
    Wu, Kuan-Lu
    Liao, Wan-Rou
    Lin, Wei-Chen
    Chen, Sung-Fang
    SEPARATIONS, 2023, 10 (04)
  • [32] Exploring Different Constituents of Milk Using Liquid Chromatography and Liquid Chromatography-Mass Spectrometry
    Gahane, Divyanshu
    Arsod, Shubham
    Dhawade, Gunjan
    Rangari, Shyam
    Gupta, Krishna
    Umekar, Milind
    JOURNAL OF ANALYTICAL CHEMISTRY, 2024, 79 (12) : 1667 - 1678
  • [33] Metabolomics for tumor marker discovery and identification based on chromatography-mass spectrometry
    Yin, Peiyuan
    Xu, Guowang
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2013, 13 (04) : 339 - 348
  • [34] Metabolomics of developing zebrafish embryos using gas chromatography- and liquid chromatography-mass spectrometry
    Huang, Shao-Min
    Xu, Fengguo
    Lam, Siew Hong
    Gong, Zhiyuan
    Ong, Choon Nam
    MOLECULAR BIOSYSTEMS, 2013, 9 (06) : 1372 - 1380
  • [35] Derivatization for liquid chromatography-mass spectrometry
    Qi, Bao-Ling
    Liu, Ping
    Wang, Qiu-Yi
    Cai, Wen-Jing
    Yuan, Bi-Feng
    Feng, Yu-Qi
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2014, 59 : 121 - 132
  • [36] Serum Metabolomics Study and Eicosanoid Analysis of Childhood Atopic Dermatitis Based on Liquid Chromatography-Mass Spectrometry
    Huang, Yan
    Chen, Guoyou
    Liu, Xinyu
    Shao, Yaping
    Gao, Peng
    Xin, Chenchen
    Cui, Zhenze
    Zhao, Xinjie
    Xu, Guowang
    JOURNAL OF PROTEOME RESEARCH, 2014, 13 (12) : 5715 - 5723
  • [37] Non-targeted metabolomics of quinoa seed filling period based on liquid chromatography-mass spectrometry
    Liu, Yongjiang
    Kong, Zhiyou
    Liu, Junna
    Zhang, Ping
    Wang, Qianchao
    Huan, Xiuju
    Li, Li
    Qin, Peng
    FOOD RESEARCH INTERNATIONAL, 2020, 137
  • [38] Endocannabinoid metabolomics: A novel liquid chromatography-mass spectrometry reagent for fatty acid analysis
    John Williams
    Lakshmipathi Pandarinathan
    Jodi Anne Wood
    Paul Vouros
    Alexandros Makriyannis
    The AAPS Journal, 8
  • [39] An Automated Workflow Composition System for Liquid Chromatography-Mass Spectrometry Metabolomics Data Processing
    Du, Xinsong
    Dastmalchi, Farhad
    Diller, Matthew A.
    Brochhausen, Mathias
    Garrett, Timothy J.
    Hogan, William R.
    Lemas, Dominick J.
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2023, 34 (12) : 2857 - 2863
  • [40] Strategies for large-scale targeted metabolomics quantification by liquid chromatography-mass spectrometry
    Zhou, Juntuo
    Yin, Yuxin
    ANALYST, 2016, 141 (23) : 6362 - 6373