共 50 条
Liquid chromatography-mass spectrometry based metabolomics investigation of different tissues of Mytilus galloprovincialis
被引:3
|作者:
James, Lea
[1
]
Gomez, Elena
[1
]
Ramirez, Gaelle
[1
]
Dumas, Thibaut
[1
]
Courant, Frederique
[1
,2
]
机构:
[1] Univ Montpellier, CNRS, IRD, Hydrosci Montpellier, Montpellier, France
[2] Hydrosci Montpellier, 15 Ave Charles Flahault, F-34090 Montpellier, France
来源:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
|
2023年
/
45卷
关键词:
Metabolomics;
Bivalve;
Gonad;
Foot;
Digestive gland;
Gill;
Soft tissue;
MUSSEL PERNA-VIRIDIS;
CYCLES;
METABOLITES;
RESPONSES;
EXPOSURE;
D O I:
10.1016/j.cbd.2022.101051
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The Mediterranean mussel (Mytilus galloprovincialis) is widely used in monitoring programs and in ecotoxico-logical studies to examine the biological effects of physicochemical parameter changes and the impact of chemical pollutants. Metabolomics has recently demonstrated high potential to gain further insight into the molecular effects of chemical exposure and the success of its application is dependent on the extent of prior metabolomics knowledge available on the target organism. Therefore, the purpose of this study was the inves-tigation of the metabolites of five different functional tissues of male and female Mediterranean mussels (digestive gland, foot, gill and gonad tissues and in the remaining soft tissues) accessible to the analysis using the most common sample preparation recommended for tissue analysis (i.e. Bligh & Dyer). Metabolic fingerprints were acquired via liquid chromatography high-resolution mass spectrometry and the identification was based on an internal database developed in the laboratory. It led to the identification of 110 metabolites, among which amino acids, carboxylic acids, purine and pyrimidine metabolites were often the most abundant. The metabolic contents of the five tissues quantitatively and qualitatively differed, with a clear distinction between male and female contents observed in the gonads and digestive glands. These results underline the importance of selecting the most suitable tissue and sex to study the impact of contamination on metabolism and the need for further research to deeper characterize the metabolome of this organism.
引用
收藏
页数:8
相关论文