3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing

被引:6
|
作者
Nicu, Raluca [1 ]
Ciolacu, Diana Elena [1 ]
Petrovici, Anca-Roxana [2 ]
Rusu, Daniela [3 ]
Avadanei, Mihaela [4 ]
Mihaila, Andreea Cristina [5 ]
Butoi, Elena [5 ]
Ciolacu, Florin [6 ]
机构
[1] Petru Poni Inst Macromol Chem, Dept Nat Polymers Bioact & Biocompatible Mat, Iasi 700487, Romania
[2] Petru Poni Inst Macromol Chem, Ctr Adv Res Bionanoconjugates & Biopolymers, Iasi 700487, Romania
[3] Petru Poni Inst Macromol Chem, Dept Phys Polymers & Polymer Mat, Iasi 700487, Romania
[4] Petru Poni Inst Macromol Chem, Dept Phys Chem Polymers, Iasi 700487, Romania
[5] Inst Cellular Biol & Pathol Nicolae Simionescu, Biopathol & Therapy Inflammat, Bucharest 050568, Romania
[6] Gheorghe Asachi Tech Univ Iasi, Dept Nat & Synthet Polymers, Iasi 700050, Romania
关键词
cellulose; dextran; polyphenols; hydrogels; controlled release; wound healing; DRUG-DELIVERY; CROSS-LINKING; IN-VITRO; DEXTRAN; HYDROGELS; BIOSYNTHESIS;
D O I
10.3390/ijms24044213
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics. In this regard, the present study aims to develop hydrogels based on cellulose and dextran (CD) and to reveal their anti-inflammatory performance. This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels. The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels. The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores. In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels. The kinetics of PFs released by hydrogels was studied according to the Korsmeyer-Peppas model, and it was observed that the transport mechanisms depend on hydrogels' composition and morphology. Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability). The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels. All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.
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页数:20
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