Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

被引：0
|
作者：
Efremova, E. P. [1 ]
Zemlyanko, O. M. [1 ,2 ]
Zhouravleva, G. A. [1 ,2 ]
机构：
[1] St Petersburg State Univ, St Petersburg 199034, Russia
[2] St Petersburg State Univ, Lab Amyloid Biol, St Petersburg 199034, Russia
来源：
MICROBIOLOGY | 2024年 / 93卷 / 02期
基金：
俄罗斯科学基金会;
关键词：
SUP45; SUP35; nonsense mutations; flow cytometry; viability; Saccharomyces cerevisiae; CHAIN RELEASE FACTORS; ERF1; ACCURACY;
D O I：
10.1134/S0026261723604244
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5-4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.
引用
收藏
页码：236 / 239
页数：4
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