MUCIN-LAYER-SECRETING IN VITRO INTESTINAL TUBE-SHAPED DEVICE WITH CRYPTIC STRUCTURE FOR BACTERIAL CO-CULTURE

被引：0

作者：

Uramoto, Shota ^{[1
]}

Tanaka, Shuma ^{[1
]}

Itai, Shun ^{[2
]}

Onoe, Hiroaki ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Tokyo, Japan

[2] Tohoku Univ, Grad Sch Biomed Engn, Sendai, Miyagi, Japan

来源：

2024 IEEE 37TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS | 2024年

关键词：

Intestinal cells; Mucin secreting; Intestinal bacteria; Co-culture; Tube-shaped device; Collagen; Electrolysis; SCAFFOLD; HEALTH;

D O I：

10.1109/MEMS58180.2024.10439293

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We propose an in vitro intestinal tube-shaped device with a secreted mucin layer that enables us to achieve stable and continuous co-culturing with bacteria. Our tube-shaped device is made of collagen hydrogel and has 3D crypt-like structures created by electrolytically generated microbubbles. Bacterial suspension can be perfused via glass/silicone tubes attached at both ends of the tube device. Supply of culture medium diffused from the outside of the tube ensures a stable culture of Caco-2 and HT29 cells on the inside of the tube. We confirmed that HT29 cells mainly secreted a mucin layer in the apical portion of cryptic structures that spatially separates the intestinal cells and bacteria as seen in vivo environments. As a result, the co-culture of intestinal cells and lactobacillus in vitro was demonstrated in the 3D crypt-like structures in our intestinal tube-shaped device.

引用

页码：485 / 488

页数：4