Different Non-Target Site Mechanisms Endow Different Glyphosate Susceptibility in Avena Species from Spain

被引：2

作者：

Vazquez-Garcia, Jose G. ^{[1
]}

Torra, Joel ^{[2
]}

Palma-Bautista, Candelario ^{[1
]}

Bastida, Fernando ^{[3
]}

Alcantara-de la Cruz, Ricardo ^{[4
]}

Portugal, Joao ^{[5
,6
]}

Jorrin-Novo, Jesus V. ^{[1
]}

De Prado, Rafael ^{[1
]}

机构：

[1] Univ Cordoba, Dept Agroforestry Plant Biochem & Mol Biol, Cordoba 14071, Spain

[2] Univ Lleida, Agrotecnio CERCA Ctr, Dept Forestry & Agr Sci & Engn, Lleida 25198, Spain

[3] Univ Huelva, Dept Ciencias Agroforestales, Huelva 21007, Spain

[4] Univ Fed Sao Carlos, Ctr Ciencias Nat, Campus Lagoa Sino, BR-18290000 Buri, Brazil

[5] Polytech Inst Beja, Biosci Dept, P-7800000 Beja, Portugal

[6] Polytech Inst Portalegre, VALORIZA Res Ctr Endogenous Resource Valorizat, P-7300555 Portalegre, Portugal

来源：

AGRONOMY-BASEL | 2023年 / 13卷 / 03期

关键词：

EPSPS; weed management strategies; plant evolution; plant resistance; pre-sowing; RYEGRASS LOLIUM-MULTIFLORUM; HERBICIDE RESISTANCE; TOLERANCE MECHANISM; DISPERSAL; FATUA;

D O I：

10.3390/agronomy13030763

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In recent decades, cereal agriculture across main producing areas in Spain has progressively adopted direct sowing, associated with an increased use of herbicides in pre-sowing. Weedy species from genus Avena have been observed after the application of glyphosate in wheat. Here, Avena fatua (two accessions), Avena byzantina and Avena sterilis subsp. sterilis, one accession each, were taxonomically characterized by a biometric study. Dose-response trials confirmed that one A. fatua accession evolved to resistance, because it was four times more resistant (R) than the others, ascribed as susceptible (S). In addition, based on LD50, A. byzantina and A. sterilis had low susceptibility to glyphosate, with 604 and 458 g ae ha(-1). Shikimic acid accumulation was able to discriminate between high susceptible (A. fatua (S)), low susceptible (A. byzantina and A. sterilis), and low resistant (A. fatua (R)) populations. On the other hand, the study revealed that A. fatua (R), A. byzantina and A. sterilis had low foliar uptake and decreased movement of glyphosate. In addition, the metabolism study showed less metabolite accumulation in A. byzantina and A. fatua (S). However, at 96 h after glyphosate application, A. fatua (R) and A. sterilis were able to convert more than 30% of glyphosate to aminomethylphosphonic acid (AMPA) and sarcosine. Enzyme basal activity and I-50 values predicted high affinity between the herbicide and the target enzyme for all accessions, thus indicating that a target-site (TS) mechanism is probably not involved in the differences in glyphosate susceptibility. In closing, non-target site (NTS) mechanisms could participate both in A. fatua R to glyphosate, and low susceptibility in A. byzantina and A. sterilis from Spain.

引用

页数：12