Effect of secretory DKK3 on circulating CD56bright natural killer cells in patients with liver cancer

被引：2
作者：
Liu, Da-Hua [1 ]
Wen, Gui-Min [2 ]
Song, Chang-Liang [3 ]
Xia, Pu [1 ,4 ]
机构：
[1] Jinzhou Med Univ, Biol Anthropol Inst, Coll Basic Med Sci, Jinzhou, Liaoning, Peoples R China
[2] Jinzhou Med Univ, Coll Nursing, Dept Community Nursing, Jinzhou, Liaoning, Peoples R China
[3] Ctr Hosp Handan, Dept Radiotherapy, Handan, Hebei, Peoples R China
[4] Jinzhou Med Univ, Biol Anthropol Inst, Jinzhou 121000, Liaoning, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 2023年 / 38卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Liver cancer; DKK3; NK cells; differentiation; cytotoxicity; NK CELLS; METHYLATION; EXPRESSION; MECHANISM; ALPHA;
D O I：
10.1177/03936155231169796
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background Liver cancer seriously threatens human health. Natural killer (NK) cells are an important part of the innate immune system and have strong anti-tumor ability. Immunotherapy based on NK cells has become a hot topic in the treatment of liver cancer. Methods In this study, we checked the serum DKK3 (sDKK3) and circulating CD56(bright) NK cells using ELISA and flow cytometry, respectively, in the blood of liver cancer patients. The effect on recombinant human DKK3 (rhDKK3) on CD56(bright) NK cells was analyzed in vitro. Results We found low levels of sDKK3 in liver cancer patients and a negative correlation between sDKK3 and circulating CD56(bright) NK cells. In addition, we found that DKK3 induced the differentiation and improved the cytotoxicity of CD56(bright) NK cells for the first time. It could be used as an agonist for NK cell-based immunotherapy. Conclusions Improving the clinical efficacy of NK cells through DKK3 will become a new strategy for cancer immunotherapy.
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页码：99 / 104
页数：6
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