The protective barrier role of satellite glial cells in sensory ganglia

被引:5
作者
Qarot, Eman [1 ,2 ]
Guan, Yun [3 ,4 ]
Hanani, Menachem [1 ,2 ,5 ]
机构
[1] Hadassah Hebrew Univ, Med Ctr, Lab Expt Surg, Jerusalem, Israel
[2] Hebrew Univ Jerusalem, Fac Med, Jerusalem, Israel
[3] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD USA
[4] Johns Hopkins Univ, Sch Med, Dept Neurol Surg, Baltimore, MD USA
[5] Hadassah Univ Hosp, Lab Expt Surg, IL-91240 Jerusalem, Israel
基金
以色列科学基金会;
关键词
ATP; dorsal root ganglion; GABA; glutamate; satellite glial cells; sensory neuron; trigeminal ganglion; DORSAL-ROOT-GANGLIA; TRIGEMINAL GANGLIA; RAT; GABA; RECEPTORS; CALCIUM; EXPRESSION; GLUTAMATE; TRANSPORTERS; NEURONS;
D O I
10.1002/glia.24511
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neurons in sensory ganglia are wrapped completely by satellite glial cells (SGCs). One putative function of SGCs is to regulate the neuronal microenvironment, but this role has received only little attention. In this study we investigated whether the SGC envelope serves a barrier function and how SGCs may control the neuronal microenvironment. We studied this question on short-term (<24 h) cell cultures of dorsal root ganglia and trigeminal ganglia from adult mice, which contain neurons surrounded with SGCs, and neurons that are not. Using calcium imaging, we measured neuronal responses to molecules with established actions on sensory neurons. We found that neurons surrounded by SGCs had a smaller response to molecules such as adenosine triphosphate (ATP), glutamate, GABA, and bradykinin than neurons without glial cover. When we inhibited the activity of NTPDases, which hydrolyze the ATP, and also when we inhibited the glutamate and GABA transporters on SGCs, this difference in the neuronal response was no longer observed. We conclude that the SGC envelope does not hinder diffusional passage, but acts as a metabolic barrier that regulates the neuronal microenvironment, and can protect the neurons and modulate their activity.
引用
收藏
页码:1054 / 1066
页数:13
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