Thermostability of native DNA bound to TiO2 nanoparticles under physiological-like conditions

被引:0
作者
Usenko, Evgeniya [1 ]
Svidzerska, Anastasiia [1 ]
Glamazda, Alexander [1 ,2 ]
Valeev, Vladimir [1 ]
Laguta, Anna [2 ,3 ]
Karachevtsev, Victor [1 ]
机构
[1] Natl Acad Sci Ukraine, B Verkin Inst Low Temp Phys & Engn, 47 Nauky Ave, UA-61103 Kharkiv, Ukraine
[2] Kharkov Natl Univ, 4 Svobody Sq, UA-61022 Kharkiv, Ukraine
[3] Aston Univ, Energy & Bioprod Res Inst, Sch Infrastruct & Sustainable Engn, Dept Chem Engn & Appl Chem, Birmingham B4 7ET, England
关键词
TiO2; nanoparticles; DNA; Nanoassemblies; Thermal denaturation; Dynamic light scattering; TITANIUM-DIOXIDE NANOPARTICLES; IN-VITRO; ADSORPTION; SURFACE; BINDING; SIZE; ZNO;
D O I
10.1007/s00396-023-05175-1
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The present work is devoted to the study of the thermostability of native DNA during binding to TiO2 nanoparticles (NPs) at various concentrations under conditions close to physiological (0.1 M Na+, pH 7) using thermal denaturation method and dynamic light scattering (DLS). The analysis of the DNA melting curves in the presence of TiO2 NPs revealed a temperature range in which the light absorption of DNA decreases. This is explained by the formation of an ordered structure of partially unwound DNA strands on the NP surface. The DNA:TiO2 NP nanoassemblies formed at the initial stages remained stable in the wide temperature range. The performed temperature-dependent DLS measurements of the DNA:TiO2 NP suspension at pH 7 have not revealed the aggregation of the DNA:TiO2 NP nanoassemblies that had been observed at pH 5.
引用
收藏
页码:23 / 30
页数:8
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