Expression of αA-crystallin (CRYAA) in vivo and in vitro models of age-related cataract and the effect of its silencing on HLEB3 cells

被引：0

作者：

Ma, Xiaoling ^{[1
]}

Nan, Yi ^{[1
]}

Huang, Can ^{[1
]}

Li, Xiangyang ^{[2
]}

Yang, Yifan ^{[1
]}

Jiang, Wenjie ^{[1
]}

Ye, Mengyi ^{[2
]}

Liu, Qian ^{[3
]}

Niu, Yang ^{[1
,2
]}

Yuan, Ling ^{[1
,4
]}

机构：

[1] Ningxia Med Univ, Minist Educ, Key Lab Ningxia Minor Med Modernizat, Yinchuan 750004, Ningxia, Peoples R China

[2] Ningxia Med Univ, Sch Tradit Chinese Med, Yinchuan 750004, Ningxia, Peoples R China

[3] Ningxia Med Univ, Sch Clin Med, Yinchuan 750004, Ningxia, Peoples R China

[4] Ningxia Med Univ, Sch Pharm, Yinchuan 750004, Ningxia Hui Aut, Peoples R China

来源：

AGING-US | 2023年 / 15卷 / 10期

基金：

中国国家自然科学基金;

关键词：

age -related cataract; CRYAA; ENDOPLASMIC-RETICULUM STRESS; NAPHTHALENE-INDUCED CATARACT; CHAPERONE ACTIVITY; LENS; APOPTOSIS; GENE;

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Aim: To investigate the expression of alpha A-crystallin (CRYAA) in age-related cataract (ARC) models and its role in lens epithelial cells (LECs). Methods: We used Flow cytometry to detect the apoptosis and cell cycle in HLEB3 cells and Real-time fluorescence quantitative polymerase chain reaction to detect the expression of CRYAA mRNA in HLEB3 and in rabbit lens. The expression of CRYAA in HLEB3 cells and rabbit lenses as well as the proteins related to apoptosis and autophagy in transfected cells were detected by western blotting. The lens structure in rabbits was investigated using hematoxylin-eosin staining. Protein thermostability assay was performed to detect the thermal stability of rabbit lens proteins. CCK- 8 assay was used to detect the viability of transfected cells, and the transfection was recorded by fluorescence photography. Results: Hydrogen peroxide can promote apoptosis and arrest the cell cycle in HLEB3 cells, and naphthalene can cause cataract formation and damage the structure of the lens in rabbits. Both ARC models can reduce the expression of CRYAA. The expression of CRYAA silencing increased apoptosis and autophagy in HLEB3 cells.

引用

页码：4498 / 4509

页数：12

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