B-GPA administration activates slow oxidative muscle signaling pathways and protects soleus muscle against the increased fatigue under 7-days of rat hindlimb suspension

被引：2

作者：

Sharlo, K. A. ^{[1
]}

Lvova, I. D. ^{[1
]}

Sidorenko, D. A. ^{[1
]}

Tyganov, S. A. ^{[1
]}

Sharlo, D. T. ^{[2
]}

Shenkman, B. S. ^{[1
]}

机构：

[1] Inst Biomed Problems RAS, Myol Lab, Moscow, Russia

[2] Bauman Moscow State Tech Univ, Fed State Budgetary Educ Inst Higher Educ, Moscow, Russia

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2023年 / 743卷

关键词：

SKELETAL-MUSCLE; EXPRESSION; FATIGABILITY; IMMOBILIZATION; ADAPTATIONS; METABOLISM; ISOFORM;

D O I：

10.1016/j.abb.2023.109647

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Unloading of slow-twitch muscles results in increased muscle fatigue and the mechanisms of this effect are poorly studied. We aimed to analyze the role of high-energy phosphates accumulation during the first week of rat hindlimb suspension plays in a fiber-type phenotype shift towards fast-type fatigable muscle fibers. Male Wistar rats were divided into 3 groups (n = 8): C -vivarium control; 7HS -7-day hindlimb suspension; 7HB -7-day hindlimb suspension with intraperitoneal injection of beta-guanidine propionic acid (8-GPA, 400 mg/kg b w). 8-GPA is a competitive inhibitor of creatine kinase and it reduces concentrations of ATP and phosphocreatine. In the 7HB group, 8-GPA treatment protected a slow-type signaling network in an unloaded soleus muscle, including MOTS-C, AMPK, PGC1 alpha and micro-RNA-499. These signaling effects resulted in a preserved soleus muscle fatigue resistance, slow-type muscle fibers percentage and mitochondrial DNA copy number under muscle unloading.

引用

页数：10