Efficacy of electroacupuncture stimulating Zusanli (ST36) and Xuanzhong (GB39) on synovial angiogenesis in rats with adjuvant arthritis

被引：0
作者：
Jianzhen, Jiang [1 ]
Xin, Zhang [1 ]
Zhenguo, Luo [2 ]
Chengguo, Su [1 ]
Haiyan, Zhou [1 ]
Yuqing, Jiang [1 ]
Xianjun, Xiao [1 ]
Yunfei, Chen [3 ]
Jun, Zhu [1 ,4 ]
机构：
[1] Chengdu Univ Tradit Chinese Med, Sch Acupuncture Moxibust & Tuina, Chengdu 610075, Peoples R China
[2] Chengdu Univ Tradit Chinese Med, Sustainabil Studies, Vocat Training Dept, Third Affiliated Hosp, Chengdu 610075, Peoples R China
[3] Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Dept Acupuncture, Shanghai 200437, Peoples R China
[4] Chengdu Univ Tradit Chinese Med, Affiliated Hosp 3, Sch Acupuncture Moxibust & Tuina, Chengdu 610075, Peoples R China
来源：
JOURNAL OF TRADITIONAL CHINESE MEDICINE | 2023年 / 43卷 / 05期
关键词：
electroacupuncture; arthritis; experimental; synovial angiogenesis; receptor; Notch1; signal transduction; ENDOTHELIAL GROWTH-FACTOR; RHEUMATOID-ARTHRITIS; PERIPHERAL-BLOOD; EXPRESSION; CELLS; MODEL; MARKERS; NOTCH1;
D O I：
10.19852/j.cnki.jtcm.20221111.002
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
OBJECTIVE: To investigate the efficacy of electroacupuncture (EA) stimulating Zusanli (ST36) and Xuanzhong (GB39) on synovial angiogenesis in rats with adjuvant arthritis (AA).METHODS: AA models were established by bilateral injection of Freund's complete adjuvant (FCA) in male Sprague-Dawley rats. Three days after injection, rats were given EA at Zusanli (ST36) and Xuanzhong (GB39) acupoints, once every other day, for 16 d. The arthritis index score, paw volume, and hematoxylin-eosin (HE) staining was performed for each animal. Angiogenesis marker cluster of differentiation 34 (CD34) expression and synovial cell apoptosis in synovial tissue were observed. The levels of Notch1, hairy and enhancer of split homolog-1 (Hes1), transforming growth factor-beta (TGF-0) and basic fibroblast growth factor (bFGF) were subsequently detected.RESULTS: We found that EA significantly decreased arthritis index scores, paw volume, and HE staining scores. EA could significantly inhibit the expression of CD34, promoting apoptosis of synovial cells in the joint synovial tissue of AA rats. The expression of Notch1 signaling pathway proteins and mRNAs (Notch1, Hes1, TGF-0, and bFGF) were markedly downregulated by EA treatment. CONCLUSIONS: These results prove that EA attenuates synovial angiogenesis by inhibiting the Notch1 signaling pathway in AA rat models. Based on our findings, we propose that EA is a promising complementary and alternative therapy in rheumatoid arthritis.(c) 2023 JTCM. All rights reserved.
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页码：955 / 962
页数：8
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