Effect of semi batch and fed batch addition of glucose on alkaline protease production: a multi-objective optimisation approach

Alkaline protease is one of the important enzymes in many industries. In this effort, semi batch addition and fed batch addition of glucose have been considered for maximisation of protease concentration in minimum fermentation time. The kinetic model of the process is validated with the experimental batch and fed batch addition of glucose from the open literature. A theoretical study has been conducted with such a validated model to check the effect of protease concentration on the semi batch addition of glucose. Based on this, multi-objective optimisation studies have been done for the simultaneous minimisation of fermentation time and maximisation of protease concentration with the relevant constraints. The elitist non-dominated sorting genetic algorithm (NSGA II) has been utilised for this purpose. The additions of glucose in semi batch mode show the potential increasing of protease concentration in at a less fermentation time as compared to the batch experimental data.