[68Ga]Ga-Ornibactin for Burkholderia cepacia complex Infection Imaging Using Positron Emission Tomography

被引：8

作者：

Bendova, Katerina ^{[1
,2
]}

Raclavsky, Vladislav ^{[3
,4
]}

Novotny, Radko ^{[3
,4
]}

Luptakova, Dominika ^{[5
]}

Popper, Miroslav ^{[1
,2
]}

Novy, Zbynek ^{[1
,2
]}

Hajduch, Marian ^{[1
,2
,6
]}

Petrik, Milos ^{[1
,2
]}

机构：

[1] Palacky Univ, Inst Mol & Translat Med, Fac Med & Dent, Olomouc 77900, Czech Republic

[2] Palacky Univ, Czech Adv Technol & Res Inst, Olomouc 77900, Czech Republic

[3] Palacky Univ, Fac Med & Dent, Dept Microbiol, Olomouc 77515, Czech Republic

[4] Univ Hosp, Olomouc 77515, Czech Republic

[5] Czech Acad Sci, Lab Mol Struct Characterizat, Inst Microbiol, Prague 4, Czech Republic

[6] Univ Hosp, Lab Expt Med, Olomouc 77900, Czech Republic

来源：

JOURNAL OF MEDICINAL CHEMISTRY | 2023年 / 66卷 / 11期

关键词：

CYSTIC-FIBROSIS; SIDEROPHORE; CENOCEPACIA; PSEUDOMONAS; ORNIBACTIN; GA-68-SIDEROPHORES; EPIDEMIOLOGY; AERUGINOSA; PET;

D O I：

10.1021/acs.jmedchem.3c00469

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Bacteria from the Burkholderia cepacia complex are generally consideredto be non-pathogenic to the healthypopulation. However, some of these species may cause serious nosocomialinfections in immunocompromised patients; as such, it is essentialto diagnose these infections rapidly so that adequate treatment canbe initiated. We report here the use of a radiolabeled siderophore,ornibactin (ORNB), for positron emission tomography imaging. We successfullyradiolabeled ORNB with gallium-68 with high radiochemical purity andproved that the resulting complex has optimal in vitro characteristics. In mice, the complex did not show excessive accumulationin organs and was excreted in the urine. We demonstrated that the[Ga-68]-Ga-ORNB complex accumulates at the site of Burkholderia multivorans infection, including pneumonia,in two animal infection models. These results suggest that [Ga-68]-Ga-ORNB is a promising tool for the diagnosis, monitoring, andevaluation of the therapeutic response to B. cepacia complex infection.

引用

页码：7584 / 7593

页数：10