Albumin Thiolation and Oxidative Stress Status in Patients with Aortic Valve Stenosis

被引:2
作者
Savini, Carlo [1 ,2 ]
Tenti, Elena [1 ]
Mikus, Elisa [1 ]
Eligini, Sonia [3 ]
Munno, Marco [3 ]
Gaspardo, Anna [3 ]
Gianazza, Erica [3 ]
Greco, Arianna [3 ]
Ghilardi, Stefania [3 ]
Aldini, Giancarlo [4 ]
Tremoli, Elena [1 ]
Banfi, Cristina [3 ]
机构
[1] Maria Cecilia Hosp, GVM Care & Res, I-48033 Cotignola, Italy
[2] Univ Bologna, Dipartimento Sci Med & Chirurg, Alma Mater Studiorum, I-40126 Bologna, Italy
[3] Ctr Cardiol Monzino IRCCS, Unit Funct Prote Metabol & Network Anal, I-20138 Milan, Italy
[4] Univ Milan, Dept Pharmaceut Sci, I-20133 Milan, Italy
关键词
albumin; oxidative stress; valve replacement; free sulfhydryl groups; serum antioxidant activity; ACETYLSALICYLIC-ACID; ASPIRIN; HEART; PLASMA; REDOX; INFLAMMATION; MECHANISMS; MANAGEMENT; MARKERS; TISSUES;
D O I
10.3390/biom13121713
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent evidence indicates that reactive oxygen species play an important causative role in the onset and progression of valvular diseases. Here, we analyzed the oxidative modifications of albumin (HSA) occurring on Cysteine 34 and the antioxidant capacity of the serum in 44 patients with severe aortic stenosis (36 patients underwent aortic valve replacement and 8 underwent a second aortic valve substitution due to a degenerated bioprosthetic valve), and in 10 healthy donors (controls). Before surgical intervention, patients showed an increase in the oxidized form of albumin (HSA-Cys), a decrease in the native reduced form (HSA-SH), and a significant reduction in serum free sulfhydryl groups and in the total serum antioxidant activity. Patients undergoing a second valve replacement showed levels of HSA-Cys, free sulfhydryl groups, and total antioxidant activity similar to those of controls. In vitro incubation of whole blood with aspirin (ASA) significantly increased the free sulfhydryl groups, suggesting that the in vivo treatment with ASA may contribute to reducing oxidative stress. We also found that N-acetylcysteine and its amide derivative were able to regenerate HSA-SH. In conclusion, the systemic oxidative stress reflected by high levels of HSA-Cys is increased in patients with aortic valve stenosis. Thiol-disulfide breaking agents regenerate HSA-SH, thus paving the way to the use these compounds to mitigate the oxidative stress occurring in the disease.
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页数:15
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共 61 条
[11]   Aspirin Inhibits the Production of Reactive Oxygen Species by Downregulating Nox4 and Inducible Nitric Oxide Synthase in Human Endothelial Cells Exposed to Oxidized Low-density Lipoprotein [J].
Chen, Beidong ;
Zhao, Jinjing ;
Zhang, Shan ;
Wu, Wei ;
Qi, Ruomei .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2012, 59 (05) :405-412
[12]   Anti-Inflammatory and Reactive Oxygen Species Suppression through Aspirin Pretreatment to Treat Hyperoxia-Induced Acute Lung Injury in NF-κB-Luciferase Inducible Transgenic Mice [J].
Chen, Chuan-Mu ;
Tung, Yu-Tang ;
Wei, Chi-Hsuan ;
Lee, Po-Ying ;
Chen, Wei .
ANTIOXIDANTS, 2020, 9 (05)
[13]   Protein oxidation and peroxidation [J].
Davies, Michael J. .
BIOCHEMICAL JOURNAL, 2016, 473 :805-825
[14]   Uncoupling the Vicious Cycle of Mechanical Stress and Inflammation in Calcific Aortic Valve Disease [J].
Dayawansa, Nalin H. ;
Baratchi, Sara ;
Peter, Karlheinz .
FRONTIERS IN CARDIOVASCULAR MEDICINE, 2022, 9
[15]   N-acetylcysteine Amide AD4/NACA and Thioredoxin Mimetic Peptides Inhibit Platelet Aggregation and Protect against Oxidative Stress [J].
Eligini, Sonia ;
Munno, Marco ;
Atlas, Daphne ;
Banfi, Cristina .
ANTIOXIDANTS, 2023, 12 (07)
[16]   N-Acetylcysteine Inhibits Platelet Function through the Regeneration of the Non-Oxidative Form of Albumin [J].
Eligini, Sonia ;
Porro, Benedetta ;
Aldini, Giancarlo ;
Colli, Susanna ;
Banfi, Cristina .
ANTIOXIDANTS, 2022, 11 (03)
[17]   A novel and automated assay for thiol/disulphide homeostasis [J].
Erel, Ozcan ;
Neselioglu, Salim .
CLINICAL BIOCHEMISTRY, 2014, 47 (18) :326-332
[18]   Antioxidant Properties of Oral Antithrombotic Therapies in Atherosclerotic Disease and Atrial Fibrillation [J].
Falco, Luigi ;
Tessitore, Viviana ;
Ciccarelli, Giovanni ;
Malvezzi, Marco ;
D'Andrea, Antonello ;
Imbalzano, Egidio ;
Golino, Paolo ;
Russo, Vincenzo .
ANTIOXIDANTS, 2023, 12 (06)
[19]   Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes [J].
Fernandez, Alma Martinez ;
Regazzoni, Luca ;
Brioschi, Maura ;
Gianazza, Erica ;
Agostoni, Piergiuseppe ;
Aldini, Giancarlo ;
Banfi, Cristina .
FREE RADICAL BIOLOGY AND MEDICINE, 2019, 144 :245-255
[20]   Aortic Valve Stenosis From Basic Mechanisms to Novel Therapeutic Targets [J].
Goody, Philip Roger ;
Hosen, Mohammed Rabiul ;
Christmann, Dominik ;
Niepmann, Sven Thomas ;
Zietzer, Andreas ;
Adam, Matti ;
Boenner, Florian ;
Zimmer, Sebastian ;
Nickenig, Georg ;
Jansen, Felix .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2020, 40 (04) :885-900