Culturing characteristics of Hanwoo myosatellite cells and C2C12 cells incubated at 37°C and 39°C for cultured meat

被引：11

作者：

Oh, Sehyuk ^{[1
]}

Park, Sanghun ^{[1
]}

Park, Yunhwan ^{[1
]}

Kim, Yun-a ^{[1
]}

Park, Gyutae ^{[1
]}

Cui, Xiangshun ^{[1
]}

Kim, Kwansuk ^{[1
]}

Joo, Seontea ^{[2
]}

Hur, Sunjin ^{[3
]}

Kim, Gapdon ^{[4
]}

Choi, Jungseok ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Anim Sci, Cheongju 28644, South Korea

[2] Gyeongsang Natl Univ, Div Appl Life Sci BK21 Four, Jinju 52852, South Korea

[3] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 17546, South Korea

[4] Seoul Natl Univ, Grad Sch Int Agr Technol, Pyeongchang 25354, South Korea

来源：

JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY | 2023年 / 65卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

Hanwoo myosatellite cell; Culture temperature; Proliferation; Differentiation; HEAT-SHOCK; IN-VITRO; ENERGY-METABOLISM; SATELLITE CELLS; STEM-CELLS; STRESS; PROLIFERATION; DIFFERENTIATION; PROTEINS; FEVER;

D O I：

10.5187/jast.2023.e10

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at dif-ferent temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37 & DEG;C and 39 & DEG;C and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37 & DEG;C proliferated better than cultured at 39 & DEG;C (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Han -woo myosatellite cells cultured at 39 & DEG;C had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37 & DEG;C. C2C12 cells cultured at 39 & DEG;C showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37 & DEG;C. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37 & DEG;C and differentiating at 39 & DEG;C are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.

引用

页码：664 / 678

页数：15