Culturing characteristics of Hanwoo myosatellite cells and C2C12 cells incubated at 37°C and 39°C for cultured meat

To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at dif-ferent temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37 & DEG;C and 39 & DEG;C and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37 & DEG;C proliferated better than cultured at 39 & DEG;C (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Han -woo myosatellite cells cultured at 39 & DEG;C had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37 & DEG;C. C2C12 cells cultured at 39 & DEG;C showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37 & DEG;C. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37 & DEG;C and differentiating at 39 & DEG;C are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.