Assessing the protection mechanisms against Enterobacter aerogenes by analyzing aggregation, adherence, antagonistic activity, and safety properties of potentially probiotic strain Lactobacillus brevis G145

被引:23
作者
Barzegar, Hassan [1 ]
Behbahani, Behrooz Alizadeh [1 ]
Mirzaei, Abbas [2 ]
Sheikhjan, Mitra Ghodsi [1 ]
机构
[1] Agr Sci & Nat Resources Univ Khuzestan, Fac Anim Sci & Food Technol, Dept Food Sci & Technol, POB 6341773637, Mollasani, Iran
[2] Agr Sci & Nat Resources Univ Khuzestan, Fac Agr Engn & Rural Dev, Dept Agr Econ, POB 6341773637, Mollasani, Iran
关键词
Probiotic potential; Caco-2; cells; Hydrophobicity; Biogenic amine; LACTIC-ACID BACTERIA; IN-VITRO; PRODUCT;
D O I
10.1016/j.micpath.2023.106175
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim of this study was to evaluate the probiotic potential of Lactobacillus brevis G145 isolated from traditional Khiki cheese, analyzing pH and bile resistance, physicochemical properties of the strain (hydrophobicity, autoand co-aggregation), cholesterol removal, hydroxyl radical scavenging activity, adhesion ability to Caco-2 cells monolayer, and adhesion competition on Enterobacter aerogenes (competition, inhibition and replacement assays). Also, DNase, haemolytic activity, biogenic amine production and antibiotic susceptibility was investigated. L. brevis G145 was resistant to acidic pHs, bile salts, and simulated gastrointestinal conditions, and showed remarkable cell surface hydrophobicity (49.56%), co-aggregation (28.90%), auto-aggregation (34.10%), adhesion (9.40%), cholesterol removal (45.50%), and antioxidant (52.19%) properties. According to the results of well diffusion agar and disc diffusion agar tests, the highest and lowest inhibition zones were accounted for Staphylococcus aureus and Enterobacter aerogenes, respectively. The isolate did not show haemolytic, DNAse, and biogenic amine production properties. It was sensitive to antibiotics erythromycin, ciprofloxacin, and chloramphenicol, and semi-sensitive to imipenem, ampicillin, nalidixic acid, and nitrofurantoin. According to the findings of probiotic tests L. brevis G145 can be used as a in the food industry.
引用
收藏
页数:8
相关论文
共 45 条
[1]   In vitro studies on probiotic and antioxidant properties of Lactobacillus brevis strain LAP2 isolated from Hentak, a fermented fish product of North-East India [J].
Aarti, Chirom ;
Khusro, Ameer ;
Varghese, Rakesh ;
Arasu, Mariadhas Valan ;
Agastian, Paul ;
Al-Dhabi, Naif Abdullah ;
Ilavenil, Soundharrajan ;
Choi, Ki Choon .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2017, 86 :438-446
[2]   Survival and stability of free and encapsulated probiotic bacteria under simulated gastrointestinal and thermal conditions [J].
Afzaal, Muhammad ;
Saeed, Farhan ;
Saeed, Muhammad ;
Azam, Muhammad ;
Hussain, Shahzad ;
Mohamed, Abdellatif A. ;
Alamri, Mohamed S. ;
Anjum, Faqir Muhammad .
INTERNATIONAL JOURNAL OF FOOD PROPERTIES, 2020, 23 (01) :1899-1912
[3]   Gamma-aminobutyric acid production by Lactobacillus brevis A3: Optimization of production, antioxidant potential, cell toxicity, and antimicrobial activity [J].
Alizadeh Behbahani, Behrooz ;
Jooyandeh, Hossein ;
Falah, Fereshteh ;
Vasiee, Alireza .
FOOD SCIENCE & NUTRITION, 2020, 8 (10) :5330-5339
[4]   Characterization of biopolymeric encapsulation system for improved survival of Lactobacillus brevis [J].
Azam, Muhammad ;
Saeed, Muhammad ;
Ahmad, Tanveer ;
Yamin, Iqra ;
Khan, Wahab Ali ;
Iqbal, Muhammad Waheed ;
Mahmood, Shahid ;
Rizwan, Muhammad ;
Riaz, Tahreem .
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2022, 16 (03) :2292-2299
[5]   Safety, probiotic properties, antimicrobial activity, and technological performance of Lactobacillus strains isolated from Iranian raw milk cheeses [J].
Barzegar, Hassan ;
Alizadeh Behbahani, Behrooz ;
Falah, Fereshteh .
FOOD SCIENCE & NUTRITION, 2021, 9 (08) :4094-4107
[6]   Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15 [J].
Behbahani, Behrooz Alizadeh ;
Noshad, Mohammad ;
Falah, Fereshteh .
MICROBIAL PATHOGENESIS, 2019, 136
[7]   New insights into probiotic mechanisms A harvest from functional and metagenomic studies [J].
Bienenstock, John ;
Gibson, Glenn ;
Klaenhammer, Todd R. ;
Walker, W. Allan ;
Neish, Andrew S. .
GUT MICROBES, 2013, 4 (02) :94-100
[8]   Safety Evaluation and Colonisation Abilities of Four Lactic Acid Bacteria as Future Probiotics [J].
Dlamini, Ziyanda C. ;
Langa, Rashwahla L. S. ;
Aiyegoro, Olayinka A. ;
Okoh, Anthony I. .
PROBIOTICS AND ANTIMICROBIAL PROTEINS, 2019, 11 (02) :397-402
[9]   Genetic diversity, safety and technological characterization of lactic acid bacteria isolated from artisanal Pico cheese [J].
Domingos-Lopes, M. F. P. ;
Stanton, C. ;
Ross, P. R. ;
Dapkevicius, M. L. E. ;
Silva, C. C. G. .
FOOD MICROBIOLOGY, 2017, 63 :178-190
[10]   Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity [J].
EL-Sayed, Abeer I. M. ;
El-Borai, Aliaa M. ;
Akl, Sara H. ;
EL-Aassar, Samy A. ;
Abdel-Latif, Mohamed S. .
SCIENTIFIC REPORTS, 2022, 12 (01)