Regulation of probe density on upconversion nanoparticles enabling high-performance lateral flow assays

被引:11
作者
Jin, Birui [1 ,3 ]
Du, Zhiguo [1 ]
Ji, Jingcheng [2 ,3 ]
Bai, Yuemeng [2 ,3 ]
Tang, Deding [2 ,3 ,4 ]
Qiao, Lihua [5 ]
Lou, Jiatao [5 ,6 ]
Hu, Jie [7 ]
Li, Zedong [2 ,3 ]
机构
[1] Xian Technol Univ, Sch Mat & Chem Engn, Xian 710021, Peoples R China
[2] Xi An Jiao Tong Univ, Key Lab Biomed Informat Engn, Minist Educ, Xian 710049, Peoples R China
[3] Xi An Jiao Tong Univ, Bioinspired Engn & Biomech Ctr BEBC, Xian 710049, Peoples R China
[4] Maanshan Teachers Coll, Maanshan 243041, Peoples R China
[5] Shanghai Jiao Tong Univ, Shanghai Chest Hosp, Dept Lab Med, Shanghai 200030, Peoples R China
[6] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Dept Lab Med, Shanghai 200080, Peoples R China
[7] Suzhou DiYinAn Biotech Co Ltd, Suzhou Innovat Ctr Life Sci & Technol, Suzhou 215129, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
Sensitivity enhancement; Lateral flow assay; Paper microfluidics; Bio-probe optimization; CRP detection; C-REACTIVE PROTEIN; QUANTITATIVE DETECTION; QUANTUM DOTS; DEVICE;
D O I
10.1016/j.talanta.2023.124327
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Upconversion nanoparticles (UCNPs)-based fluorescence probes have shown great potential in point-of-care testing (POCT) applications, due to UCNPs' features of high photostability and background-free fluorescence. Ceaseless improvements of UCNPs-probes have been carried out to increase detection sensitivity and to broaden detection range of UCNPs-based POCT. In this paper, we optimized UCNPs-probes by regulating probe density. The optimization was verified by a traditional lateral flow assay (LFA) platform for C-reactive protein (CRP) detection. Further, the optimized UCNPs-LFA integrating with a home-made benchtop fluorescence analyzer holds the capability to achieve high-performance POCT. Finally, nearly a 20 times sensitivity enhancement with a limit of detection of 0.046 ng/mL and a broad detection range of 0.2-300 ng/mL for CRP detection was ob-tained. Moreover, the optimized UCNPs-LFA was applied to detecting CRP in clinical serum samples and the detection results were consistent with the clinical test, validating its clinical practicability. The proposed opti-mization method is also expected to optimize other nanoparticles-based bio-probes for wider POCT application.
引用
收藏
页数:8
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