Evaluating DNA barcoding using cpDNA matK and rbcL for species identification and phylogenetic analysis of Prunus armeniaca L. (Rosaceae) genotypes

被引：4

作者：

Sevindik, Emre ^{[1
]}

Korkom, Yunus ^{[2
]}

Murathan, Zehra Tugba ^{[3
]}

机构：

[1] Adnan Menderes Univ, Fac Agr, Dept Agr Biotechnol, South Campus, Aydin, Turkiye

[2] Adnan Menderes Univ, Fac Agr, Dept Plant Protect, Aydin, Turkiye

[3] Malatya Turgut Ozal Univ, Battalgazi Vocat Sch, Malatya, Turkiye

来源：

GENETIC RESOURCES AND CROP EVOLUTION | 2024年 / 71卷 / 05期

关键词：

matK; rbcL; Phylogenetic; Prunus armeniaca; Turkiye; MOLECULAR PHYLOGENY; SPACER; NRDNA; WILD;

D O I：

10.1007/s10722-023-01748-9

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In this study, DNA barcoding and phylogenetic analysis of five Prunus armeniaca L. genotypes (Cataloglu, Hacihaliloglu, Hasanbey, Hudayi, and Kabaasi) grown in Malatya/Turkiye were conducted using chloroplast DNA (cpDNA) matK and rbcL regions. The cpDNA matK region was amplified using matK472F and matK1248R primers, while the rbcL region was amplified with rbcLaF and rbcLaR primers. The matK and rbcL sequences were utilized to assess nucleotide ratios, genetic distance, and nucleotide diversity (p). The neighbor- joining (NJ) tree including other Prunus species from NCBI was created. In addition, physiochemical and 3-D analysis of matK and rbcL proteins were performed. As a result, p = 0.000549 for matK sequence and p = 0.002657 for rbcL sequence were determined. NJ (neighbor joining) phylogenetic trees formed with both matK and rbcL sequences were found to be compatible with each other. The matK and rbcL gene regions were found to be suitable for phylogenetic analysis.

引用

页码：1825 / 1835

页数：11

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