Autophagy activation, histopathological damage, and altered renal epithelial sodium channel and Na plus ,K plus -ATPase gene expression in offspring kidney after in utero exposure to allethrin

被引:0
|
作者
Alqahtani, Wadha [1 ]
Mufti, Afoua [2 ]
Aldawood, Nouf [3 ]
Alshamrani, Abdullah [1 ]
Nahdi, Saber [1 ]
Aldahmash, Waleed [1 ]
Jalouli, Maroua [4 ]
Feriani, Anouar [2 ]
Mansour, Lamjed [1 ]
Tlili, Nizar [5 ]
Alwasel, Saleh [1 ]
Harrath, Abdel Halim [1 ]
机构
[1] King Saud Univ, Coll Sci, Zool Dept, Riyadh 11451, Saudi Arabia
[2] Univ Gafsa, Lab Biotechnol & Biomonitoring Environm & Oasis Ec, Gafsa 2112, Tunisia
[3] Princess Nourah bint Abdulrahman Univ, Coll Sci, Dept Biol, BOB 84428, Riyadh 11671, Saudi Arabia
[4] Imam Mohammad Ibn Saud Islamic Univ IMSIU, Coll Sci, Dept Biol, Riyadh 11623, Saudi Arabia
[5] Univ Carthage, Inst Super Sci & Technol Environm Borj Cedria, Tunis, Tunisia
关键词
Pyrethroid insecticide; Allethrin; Nephrotoxicity; Autophagy; Histopathology; APOPTOSIS; CADMIUM;
D O I
10.1016/j.jksus.2023.102575
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The goal of the current study was to determine the effects of the allethrin, as a pyrethroid-based insec-ticide, on kidney function in rat offspring. Sixty-day-old Wistar albino female rats were randomly allo-cated to three groups: control group and two allethrin-treated groups that were intragastrically administered with 34.2 or 68.5 mg/kg b.w. of allethrin starting from the day 6 of pregnancy until delivery. The results revealed that the high dose of allethrin (68.5 mg/kg) reduced the number of glomeruli. Results showed that the glomeruli diameter in animals treated with 34.2 mg/kg of allethrin significantly decreased when compared to the controls (p < 0.001) whereas at the dose of 68.5 mg/kg the glomeruli diameter was significantly higher than those of the control group (p < 0.05). Interestingly, the expression of the autophagic markers Beclin-1 and LC-3 was increased when compared to control indicating an induction of the autophagic mechanism. Furthermore, allethrin administration downregulated in a dose-dependent manner the expression of the epithelial sodium channel (ENaC) gene. The mRNA levels of Na+/K+-ATPase gene were significantly higher in the group treated with the low dose and significantly lower with the high dose, when compared to control. However, there was no effect of allethrin on the gene expression of NHE3 in both treated groups. Taken together, the findings of this study suggested that the exposure to allethrin might contribute to the nephrotoxicity by affecting the number and the diam-eter of glomeruli, causing histopathological alterations and inducing autophagic-related markers and affecting the mRNA levels of ENaC and Na+/ K + ATPase genes. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页数:7
相关论文
共 4 条
  • [1] Maternal Exposure to Acephate Caused Nephrotoxicity in Adult Offspring Rats Mediated by Excessive Autophagy Activation, Oxidative Stress Induction, and Altered Epithelial Sodium Channel and Na+/K+-ATPase Gene Expression
    Mufti, Afoua
    Jalouli, Maroua
    Nahdi, Saber
    Tlili, Nizar
    Alqahtani, Wadha
    Mansour, Lamjed
    Alwasel, Saleh
    Harrath, Abdel Halim
    BIOLOGY-BASEL, 2023, 12 (02):
  • [2] cAMP improves deficient sodium channel and Na plus K plus ATPase expression and function in vivo and in vitro
    Falck, AJ
    Castro, R
    Seidner, SR
    Petershack, JA
    Mendoza, YM
    Crichton, CA
    Mustafa, SB
    PEDIATRIC RESEARCH, 2002, 51 (04) : 396A - 396A
  • [3] Bisphenol A impairs renal function by reducing Na plus /K plus -ATPase and F-actin expression, kidney tubule formation in vitro and in vivo
    Yoo, Min Heui
    Lee, Seung-Jin
    Kim, Woojin
    Kim, Younhee
    Kim, Yong-Bum
    Moon, Kyoung-Sik
    Lee, Byoung-Seok
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022, 246
  • [4] Soluble Adenylyl Cyclase in Vascular Endothelium Gene Expression Control of Epithelial Sodium Channel-α, Na+/K+-ATPase-α/β, and Mineralocorticoid Receptor
    Schmitz, Boris
    Nedele, Johanna
    Guske, Katrin
    Maase, Martina
    Lenders, Malte
    Schelleckes, Michael
    Kusche-Vihrog, Kristina
    Brand, Stefan-Martin
    Brand, Eva
    HYPERTENSION, 2014, 63 (04) : 753 - 761