Crossprotection induced by virus-like particles containing influenza dual-hemagglutinin and M2 ectodomain

被引：0
|
作者：
Mao, Jie [1 ]
Eom, Gi-Deok [1 ]
Yoon, Keon-Woong [1 ]
Kim, Min-Ju [1 ]
Chu, Ki-Back [2 ]
Kang, Hae-Ji [3 ]
Quan, Fu-Shi [2 ,4 ]
机构：
[1] Kyung Hee Univ, Grad Sch, Dept Biomed Sci, Seoul 02447, South Korea
[2] Kyung Hee Univ, Biomed Sci Inst, Core Res Inst CRI, Med Res Ctr Bioreact React Oxygen Species, Seoul 02447, South Korea
[3] Georgia State Univ, Inst Biomed Sci, Ctr Translat Antiviral Res, Atlanta, GA 30303 USA
[4] Kyung Hee Univ, Sch Med, Dept Med Zool, Seoul 02447, South Korea
来源：
NANOMEDICINE | 2024年 / 19卷 / 09期
基金：
新加坡国家研究基金会;
关键词：
hemagglutinin; influenza virus; M2e5x; universal vaccine; virus-like particles; CROSS-PROTECTION; VACCINE; MICE; H1N1; INFECTION; IMMUNITY; SUBTYPES;
D O I：
10.2217/nnm-2023-0353
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: To develop an effective universal vaccine against antigenically different influenza viruses. Materials & methods: We generated influenza virus-like particles (VLPs) expressing the H1 and H3 antigens with or without M2e5x. VLP-induced immune responses and crossprotection against H1N1, H3N2 or H5N1 viruses were assessed to evaluate their protective efficacy. Results: H1H3M2e5x immunization elicited higher crossreactive IgG antibodies than H1H3 VLPs. Upon challenge, both VLPs enhanced lung IgG, IgA and germinal center B-cell responses compared with control. While these VLPs conferred protection, H1H3M2e5x showed greater lung viral load reduction than H1H3 VLPs with minimal body weight loss. Conclusion: Utilizing VLPs containing dual-hemagglutinin, along with M2e5x, can be a vaccination strategy for inducing crossprotection against influenza A viruses. [GRAPHICS] .
引用
收藏
页码：741 / 754
页数：14
相关论文
共 50 条
  • [41] Plant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages
    Makarkov, Alexander I.
    Chierzi, Sabrina
    Pillet, Stephane
    Murai, Keith K.
    Landry, Nathalie
    Ward, Brian J.
    VACCINE, 2017, 35 (35) : 4629 - 4636
  • [42] Vaccination induced antibodies to recombinant avian influenza A virus M2 protein or synthetic M2e peptide do not bind to the M2 protein on the virus or virus infected cells
    Swinkels, Willem J. C.
    Hoeboer, Jeroen
    Sikkema, Reina
    Vervelde, Lonneke
    Koets, Ad P.
    VIROLOGY JOURNAL, 2013, 10
  • [43] Virus-Like Particles Are a Superior Platform for Presenting M2e Epitopes to Prime Humoral and Cellular Immunity against Influenza Virus
    Kim, Ki-Hye
    Kwon, Young-Man
    Lee, Young-Tae
    Kim, Min-Chul
    Hwang, Hye Suk
    Ko, Eun-Ju
    Lee, Youri
    Choi, Hyo-Jick
    Kang, Sang-Moo
    VACCINES, 2018, 6 (04):
  • [44] Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses
    Wu, Peipei
    Lu, Jihu
    Zhang, Xuehua
    Mei, Mei
    Feng, Lei
    Peng, Daxin
    Hou, Jibo
    Kang, Sang-Moo
    Liu, Xiufan
    Tang, Yinghua
    FRONTIERS IN IMMUNOLOGY, 2017, 8
  • [45] Mono- and quadri-subtype virus-like particles (VLPs) containing H10 subtype elicit protective immunity to H10 influenza in a ferret challenge model
    Pushko, Peter
    Sun, Xiangjie
    Tretyakova, Irina
    Hidajat, Rachmat
    Pulit-Penaloza, Joanna A.
    Belser, Jessica A.
    Maines, Taronna R.
    Tumpey, Terrence M.
    VACCINE, 2016, 34 (44) : 5235 - 5242
  • [46] Enhanced Immunogenicity of an Influenza Ectodomain Matrix-2 Protein Virus-like Particle (M2e VLP) Using Polymeric Microparticles for Vaccine Delivery
    Gomes, Keegan Braz
    Menon, Ipshita
    Bagwe, Priyal
    Bajaj, Lotika
    Kang, Sang-Moo
    D'Souza, Martin J.
    VIRUSES-BASEL, 2022, 14 (09):
  • [47] M2e-Displaying Virus-Like Particles with Associated RNA Promote T Helper 1 Type Adaptive Immunity against Influenza A
    Ibanez, Lorena Itati
    Roose, Kenny
    De Filette, Marina
    Schotsaert, Michael
    De Sloovere, Jessica
    Roels, Stefan
    Pollard, Charlotte
    Schepens, Bert
    Grooten, Johan
    Fiers, Walter
    Saelens, Xavier
    PLOS ONE, 2013, 8 (03):
  • [48] Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data
    Falynskova, Irina N.
    Egorov, Andrej Yu
    Poddubikov, Alexander, V
    Vartanova, Nune O.
    Kartashova, Nadezhda P.
    Glubokova, Ekaterina A.
    Mkhitarov, Vladimir A.
    Dzhalilova, Dzhuliya S.
    Makarova, Olga, V
    Leneva, Irina A.
    VOPROSY VIRUSOLOGII, 2020, 65 (03) : 150 - 158
  • [49] Protection induced by malaria virus-like particles containing codon-optimized AMA-1 of Plasmodium berghei
    Dong-Hun Lee
    Ki-Back Chu
    Hae-Ji Kang
    Su-Hwa Lee
    Manika Chopra
    Hyo-Jick Choi
    Eun-Kyung Moon
    Kyung-Soo Inn
    Fu-Shi Quan
    Malaria Journal, 18
  • [50] Virus-Like Particle Vaccine Containing Hemagglutinin Confers Protection against 2009 H1N1 Pandemic Influenza
    Hossain, M. Jaber
    Bourgeois, Melissa
    Quan, Fu-Shi
    Lipatov, Aleksandr S.
    Song, Jae-Min
    Chen, Li-Mei
    Compans, Richard W.
    York, Ian
    Kang, Sang-Moo
    Donis, Ruben O.
    CLINICAL AND VACCINE IMMUNOLOGY, 2011, 18 (12) : 2010 - 2017
12345