A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media.

被引：2

作者：

Vozniuk, Oleksandra ^{[3
,4
]}

Kejik, Zdenek ^{[1
,2
,3
,4
]}

Vesela, Katerina ^{[1
,2
,3
]}

Skalickova, Marketa ^{[1
,2
,3
]}

Novotny, Petr ^{[1
,2
,3
]}

Hromadka, Robert ^{[1
,2
,3
]}

Hajduch, Jan ^{[1
,2
,3
]}

Martasek, Pavel ^{[1
,2
]}

Jakubek, Milan ^{[1
,2
,3
,4
]}

机构：

[1] Charles Univ Prague, Fac Med 1, Dept Paediat & Inherited Metab Disorders, Ke Karlovu 455-2, Prague 12808, Czech Republic

[2] Gen Univ Hosp Prague, Ke Karlovu 455-2, Prague 12808, Czech Republic

[3] Charles Univ Prague, Fac Med 1, BIOCEV, Vestec 25220, Czech Republic

[4] Univ Chem & Technol, Fac Chem Engn, Dept Analyt Chem, Prague 16628, Czech Republic

来源：

CHEMISTRYOPEN | 2024年 / 13卷 / 03期

关键词：

HPLC/UV; ketoprofen; cellular medium; IN-VITRO; TABLET;

D O I：

10.1002/open.202300147

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 mu g/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro. A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media. Separation was performed using a gradient on a C18 column with a mobile phase of acetonitrile and acidified miliQ water. The method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, as well as for robustness. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.image

引用

页数：9

共 27 条

[1] Pilot study of relative bioavailability of two oral formulations of ketoprofen 25 mg in healthy subjects. A fast-dissolving lyophilized tablet as compared to immediate release tablet [J].