Identification and Quantification of Antibiotic Residues and Evaluation of Microbial Resistance to Antibiotics in Huatanay River Waters in Peru

被引:2
作者
de Loayza, Tatiana Del Castillo [1 ]
Maldonado, Ingrid [2 ]
Vilca, Franz Zirena [3 ]
机构
[1] UNSAAC, Fac Ciencias Salud, Dept Acad Farm, Cuzco, Peru
[2] Univ Nacl Altiplano Puno, Escuela Posgrad, Programa Doctorado Ciencia Tecnol & Medio Ambiente, Ave Floral 1153, Puno, Peru
[3] Univ Nacl Moquegua, Lab Contaminantes Organ & Ambiente IINDEP, Urb Ciudad Jardin Pacocha, Peru
来源
POLLUTION | 2023年 / 9卷 / 03期
关键词
Bacteria resistance; Chromatography; Emerging pollutants; Environmental Health; Pollution; Solid phase xtraction; wastewater; WASTE-WATER; TRANSFORMATION PRODUCTS; CONSTRUCTED WETLANDS; SALMONELLA; CIPROFLOXACIN; ENVIRONMENT; EFFLUENT; REMOVAL; CYCLE; RISK;
D O I
10.22059/poll.2023.352228.1718
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The Huatanay River in Cusco-Peru, is affected by wastewater discharges along its course. In order to evaluate this impact, we evaluate antibiotic residues and their impact on the increase of bacterial resistance in the city of Cusco treatment plant. For this purpose, water samples from the influent and effluent of the treatment plant were analyzed by chromatographic methods; additionally, sensitivity tests were performed with three bacterial strains (Escherichia coli, Salmonella sp., and Klebsiella sp.), which were isolated from the same place. Six antibiotic residues were identified (ceftriaxone, amoxicillin, trimethoprim, sulfamethoxazole, dicloxacillin, and lincomycin). Those found in the highest concentration were: amoxicillin (91495 and 0 & mu;g/L) and lincomycin (33970 and 10800 & mu;g/L) in the influent and effluent, respectively. There is more resistance in the effluent than the influent in the case of E. coli shows resistance in the effluent to cephalexin (30 & mu;g) and azithromycin (15 & mu;g). Salmonella sp. is resistant to amoxicillin (15 & mu;g), dicloxacillin (1 & mu;g), lincomycin (2 & mu;g), ceftriaxone (30 & mu;g), cephalexin (30 & mu;g), and ciprofloxacin (5 & mu;g). Finally, Klebsiella sp. is sensitive to ceftriaxone (30 & mu;g), amoxicillin (15 & mu;g), and cephalexin (30 & mu;g). This confirms that the antibiotic residues contained in the wastewater of Cusco generate resistance in the isolated bacteria.
引用
收藏
页码:1236 / 1250
页数:15
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