Anticancer activity of Green synthesized selenium nanoparticles from Garcinia Crude extract MCF-7 Breast cancer cells

Background: Nano-biotechnology has evolved and plays a crucial role in the detection and treatment of many diseases in the current scenario. An eco-friendly and efficient method (photoinduced) has been used for green synthesis of stable selenium nanoparticles (SeNPs) using aqueous extract as a reducing and stabilizing agent, derived from fruit extract of garcinia mangostana.Aim: The aim of this study was to evaluate the anticancer activity of green synthesized GM selenium nanoparticles (Se-NPs) against breast cancer cells (MCF-7).Methods And Materials: The preparation of selenium-induced Garcinia Mangostana nanoparticles were examined by ultraviolet-visible spectroscopy with a wavelength of range from 25-650nm. The selenium nanoparticle pellet was stored in sealed vials after being calcined for two hours at 70 degrees C in a hot air oven.In-vitro antitumor activity of Se-NPs was evaluated by MTT assay. Inverted microscope was used to evaluate the morphology of the cells.Results: The synthesized Selenium Nanoparticles have anticancer against MCF-7 cell lines. The cytotoxicity study exhibited a dose-dependent effect against breast cancer cells (MCF-7) using MTT assay, the inhibitory concentration (IC50) was found to be 50 mu g/mL . Inverted phase contrast microscope showed low viability of cell at a concentration of 60ul in the breast cancer cell line.Conclusion: In conclusion, the results of this study demonstrated that Garcinia Mangostana induced selenium Nanoparticles extract may be a potential therapeutic agent for human breast cancer treatment.