Evaluation of bone-like apatite biomineralization on biomimetic graphene oxide/hydroxyapatite nanocomposite

Graphene oxide/Hydroxy apatite (GO/HAp) nanocomposites are fabricated with the different ratios of HAp by hydrothermal process. In this study, the additive influence of the different ratios of HAp on bone-like HAp crystal growth was investigated. We tested the influences of the different ratios of HAp on the bioactivity ability of GO/ HAp nanocomposite via biomineralization assay in a simulated body fluid (SBF) solution. The formation of GO/ HAp nanocomposite was confirmed by Fourier Transform Infrared (FTIR), X-ray Diffraction (XRD), and Trans-mission Electron Microscopy (TEM). Here, GO/HAp nanocomposite was synthesized with three weight per-centages of HAp (low, mid, and, high) and tested to induce biomineralization in a simulated body fluid (SBF) solution. To observe the mineralization of bone-like apatite on the GO nanosheet's surface, Field Emission Scanning Electron Microscopy (FE-SEM) and Energy-dispersive X-ray spectrum (EDX) elemental mapping were utilized. Biomineralization test results exhibited that the GO with a higher amount of HAp accelerated the deposition of like apatite structures in an in vitro model.