MORN2 regulates the morphology and energy metabolism of mitochondria and is required for male fertility in mice

被引:2
作者
Liu, Yining [1 ,2 ,3 ,4 ]
Li, Tongtong [1 ,2 ,3 ,4 ]
Shi, Mingze [1 ,2 ,3 ,4 ]
Wan, Yanling [1 ,2 ,3 ,4 ]
Li, Hanzhen [1 ,2 ,3 ,4 ]
Zhang, Mingyu [1 ,2 ,3 ,4 ]
Wang, Ziqi [1 ,2 ,3 ,4 ]
Wang, Shiyu [1 ,2 ,3 ,4 ]
Lv, Yue [5 ,6 ]
Lu, Gang [1 ,2 ,3 ,4 ,6 ]
Liu, Hongbin [1 ,2 ,3 ,4 ,6 ]
Zhang, Haobo [1 ,2 ,3 ,4 ,7 ]
Huang, Tao [1 ,2 ,3 ,4 ]
机构
[1] Shandong Univ, Ctr Reprod Med, Jinan 250012, Shandong, Peoples R China
[2] Shandong Univ, Key Lab Reprod Endocrinol, Minist Educ, Jinan 250012, Shandong, Peoples R China
[3] Shandong Univ, Shandong Prov Clin Med Res Ctr Reprod Hlth, Jinan 250012, Shandong, Peoples R China
[4] Shandong Technol Innovat Ctr Reprod Hlth, Jinan 250012, Shandong, Peoples R China
[5] Shandong First Med Univ, Shandong Prov Hosp, Shandong Key Lab Reprod Med, Jinan 250012, Shandong, Peoples R China
[6] Chinese Univ Hong Kong, Sch Biomed Sci, CUHK SDU Joint Lab Reprod Genet, Hong Kong, Peoples R China
[7] Shandong Univ, Hosp 2, Cheeloo Coll Med, Ctr Reprod Med, Jinan 250012, Shandong, Peoples R China
基金
国家重点研发计划;
关键词
Asthenospermia; Sperm motility; Mitochondrial sheath; Energy metabolism; MORN2; SPERM MOTILITY; SHEATH; PHOSPHORYLATION;
D O I
10.1186/s12967-024-05010-3
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
BackgroundMitochondria produce adenosine triphosphate through respiratory activities to power sperm differentiation and motility, and decreased mitochondrial respiratory activity can result in poor sperm motility and asthenospermia. The mitochondrial sheath is a component of the mid-piece of the sperm flagellum, and dysfunction of the sheath can reduce sperm motility and cause male infertility. The membrane occupation and recognition nexus-motif protein 2 (MORN2) is testis enriched in mice, and the MORN motif was reported to play a role in the regulation of bioelectrical signal homeostasis in cardiomyocytes.MethodsWe generated Morn2-/- mice using CRISPR/Cas9 and evaluated the potential functions of MORN2 in spermiogenesis through histological analysis, fertility examination, RT-PCR, CASA, immunofluorescence, TUNEL, electron microscopy analysis, mitochondrial energy metabolism analysis, etc.ResultsThe Morn2-/- mice were infertile, and their sperm showed severe motility defects. Morn2-/- sperm also had abnormal morphology characterized by bent heads, aberrant mitochondrial sheath formation, lower mitochondrial membrane potential, higher levels of reactive oxygen species, and decreased mitochondrial respiratory activity.ConclusionsOur study demonstrates that MORN2 is essential for male fertility and indicates that MORN2 functions in mitochondrial sheath formation and regulates mitochondrial respiratory activity.
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页数:16
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