Prevalence of Enterococcus spp. and the Whole-Genome Characteristics of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Free-Living Birds in Poland

被引：11

作者：

Kwit, Renata ^{[1
]}

Zajac, Magdalena ^{[1
]}

Smialowska-Weglinska, Aleksandra ^{[1
]}

Skarzynska, Magdalena ^{[1
]}

Bomba, Arkadiusz ^{[2
]}

Lalak, Anna ^{[1
]}

Skrzypiec, Ewelina ^{[1
]}

Wojdat, Dominika ^{[1
]}

Koza, Weronika ^{[1
]}

Mikos-Wojewoda, Emilia ^{[1
]}

Pasim, Paulina ^{[1
]}

Skora, Milena ^{[1
,4
]}

Polak, Marcin ^{[3
]}

Wiacek, Jaroslaw ^{[3
]}

Wasyl, Dariusz ^{[1
,2
]}

机构：

[1] Natl Vet Res Inst PIWet, Dept Microbiol, PL-24100 Pulawy, Poland

[2] Natl Vet Res Inst PIWet, Dept Omic Anal, PL-24100 Pulawy, Poland

[3] Maria Curie Sklodowska Univ UMCS, Inst Biol Sci, Dept Zool & Nat Protect, PL-20033 Lublin, Poland

[4] Univ Lodz, Fac Biol & Environm Protect, Dept Oncobiol & Epigenet, Biobank Lab, PL-91402 Lodz, Poland

来源：

PATHOGENS | 2023年 / 12卷 / 06期

关键词：

wild birds; Enterococcus faecium; Enterococcus faecalis; antimicrobial resistance; WGS; ANTIBIOTIC-RESISTANCE; WILD BIRDS; ANTIMICROBIAL RESISTANCE; VIRULENCE FACTORS; FECAL ENTEROCOCCI; ESCHERICHIA-COLI; ANIMAL ORIGIN; DIVERSITY; VANA; GENES;

D O I：

10.3390/pathogens12060836

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterococci as opportunistic bacteria are important for human health. Due to the prevalence and ease of acquisition and transfer of their genes, they are an excellent indicator of environmental contamination and the spread of antimicrobial resistance. The aim of the study was to assess the prevalence of Enterococcus spp. in wild birds in Poland, determination of antimicrobial susceptibility and WGS analysis of Enterococcus (E.) faecium and E. faecalis. For this purpose, 138 samples from various species of free-living birds were tested, with 66.7% positive results. Fourteen species were detected, with E. faecalis being the most common, followed by E. casseliflavus and E. hirae. In antimicrobial susceptibility testing, 10.0% of E. faecalis and 50.0% of E. faecium showed resistance to one antimicrobial agent, in addition the MDR phenotype which was found in one E. faecium. The most common resistance phenotype included tetracycline and quinupristin/dalfopristin. The WGS analysis confirmed the significant advantage of the virulence gene diversity of E. faecalis strains over E. faecium. In addition, plasmid replicons were found in 42.0% of E. faecalis and 80.0% of E. faecium. The obtained results confirm free-living birds can be a reservoir of Enterococcus spp. with a considerable zoonotic potential.

引用

页数：15

共 48 条

[1] Comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and vegetable foods, water and soil, and clinical samples [J].

Abriouel, Hikmate ;

Ben Omar, Nabil ;

Molinos, Antonio Cobo ;

Lopez, Rosario Lucas ;

Grande, M. Jose ;

Martinez-Viedma, Pilar ;

Ortega, Elena ;

Canamero, Magdalena Martinez ;

Galvez, Antonio .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 123 (1-2) :38-49

[2] Antimicrobial resistance and genetic lineages of faecal enterococci of wild birds: Emergence of vanA and vanB2 harbouring Enterococcus faecalis [J].

Ben Yahia, Houssem ;

Chairat, Sarra ;

Hamdi, Nabil ;

Gharsa, Haythem ;

Ben Sallem, Rym ;

Ceballos, Sara ;

Torres, Carmen ;

Ben Slama, Karim .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2018, 52 (06) :936-941

[3] Enterococcal leucine-rich repeat-containing protein involved in virulence and host inflammatory response [J].

Brinster, Sophie ;

Posteraro, Brunella ;

Bierne, Helene ;

Alberti, Adriana ;

Makhzarni, Sarnira ;

Sanguinetti, Maurizio ;

Serror, Pascale .

INFECTION AND IMMUNITY, 2007, 75 (09) :4463-4471

[4] Antimicrobial-Resistant Enterococcus spp. in Wild Avifauna from Central Italy [J].

Cagnoli, Giulia ;

Bertelloni, Fabrizio ;

Interrante, Paolo ;

Ceccherelli, Renato ;

Marzoni, Margherita ;

Ebani, Valentina Virginia .

ANTIBIOTICS-BASEL, 2022, 11 (07)

[5] Antimicrobial Resistance, Virulence Genes, and Biofilm Formation Capacity AmongEnterococcus speciesFrom Yaks in Aba Tibetan Autonomous Prefecture, China [J].

Cui, Pengfei ;

Feng, Lan ;

Zhang, Lan ;

He, Juan ;

An, Tianwu ;

Fu, Xue ;

Li, Cui ;

Zhao, Xiaodong ;

Zhai, Yaru ;

Li, Hao ;

Yan, Wenjun ;

Li, Huade ;

Luo, Xiaolin ;

Lei, Changwei ;

Wang, Hongning ;

Yang, Xin .

FRONTIERS IN MICROBIOLOGY, 2020, 11

[6] Antibiotic Susceptibility and Virulence Genes in Enterococcus Isolates from Wild Mammals Living in Tuscany, Italy [J].

Dec, Marta ;

Stepien-Pysniak, Dagmara ;

Gnat, Sebastian ;

Fratini, Filippo ;

Urban-Chmiel, Renata ;

Cerri, Domenico ;

Winiarczyk, Stanislaw ;

Turchi, Barbara .

MICROBIAL DRUG RESISTANCE, 2020, 26 (05) :505-519

[7]

Denys A., 2009, MED DOSW, V015, P125

[8] Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp.: 2.: Pheno- and genotypic criteria [J].

Domig, KJ ;

Mayer, HK ;

Kneifel, W .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 88 (2-3) :165-188

[9] Prevalence and conservation of ebp genes in Enterococcus faecalis originated from animals [J].

Dong, Jiajun ;

Zhang, Liujun ;

He, Hengxu ;

Jin, Yue ;

Wang, Yabin ;

Chen, Liying .

JOURNAL OF APPLIED MICROBIOLOGY, 2022, 132 (04) :3293-3301

[10] INDUCIBLE TRANSFER OF CONJUGATIVE TRANSPOSON TN1545 FROM ENTEROCOCCUS-FAECALIS TO LISTERIA-MONOCYTOGENES IN THE DIGESTIVE TRACTS OF GNOTOBIOTIC MICE [J].

DOUCETPOPULAIRE, F ;

TRIEUCUOT, P ;

DOSBAA, I ;

ANDREMONT, A ;

COURVALIN, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (01) :185-187

← 1 2 3 4 5 →