Fish Oil Ameliorates Vibrio parahaemolyticus Infection in Mice by Restoring Colonic Microbiota, Metabolic Profiles, and Immune Homeostasis

被引：5

作者：

Deng, Yijia ^{[1
,2
]}

Wang, Rundong ^{[1
,2
]}

Li, Xuepeng ^{[2
]}

Tan, Xiqian ^{[2
]}

Zhang, Yuhao ^{[1
]}

Gooneratne, Ravi ^{[3
]}

Li, Jianrong ^{[1
,2
]}

机构：

[1] Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

[2] Bohai Univ, Coll Food Sci & Engn, Jinzhou 121013, Peoples R China

[3] Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 18期

基金：

中国国家自然科学基金; 中国博士后科学基金; 国家重点研发计划;

关键词：

fish oil; Vibrio parahaemolyticus; immune homeostasis; microbiota diversity; gut metabolites; SUPPLEMENTATION; PATHOGENESIS; SALMONELLA; MECHANISMS; MODELS;

D O I：

10.1021/acs.jafc.2c08559

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The effect of fish oil (FO) on colonic function, immunity, and microbiota was investigated in Vibrio parahaemolyticus (Vp)-infected C57BL/6J mice. Mice intragastrically presupplemented with FO (4.0 mg) significantly reduced Vp infection as evidenced by stabilizing body weight and reducing disease activity index score and immune organ ratios. FO minimized colonic pathological damage, strengthened the mucosal barrier, and sustained epithelial permeability by increasing epithelial crypt depth, goblet cell numbers, and tight junctions and inhibiting colonic collagen accumulation and fibrosis protein expression. Mechanistically, FO enhanced immunity by decreasing colonic CD3+ T cells, increasing CD4+ T cells, downregulating the TLR4 pathway, reducing interleukin-17 (IL-17) and tumor necrosis factor-alpha, and increasing immune cytokine IL-4 and interferon-gamma levels. Additionally, FO maintained colonic microbiota eubiosis by improving microbial diversity and boosting Clostridium, Akkermansia, and Roseburia growth and their derived propionic acid and butyric acid levels. Collectively, FO alleviated Vp infection by enriching beneficial colonic microbiota and metabolites and restoring immune homeostasis.

引用

页码：6920 / 6934

页数：15

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