Hollow Microneedle-based Plasmonic Sensor for on Patch Detection of Molecules in Dermal Interstitial Fluid

被引：25

作者：

Miranda, Bruno ^{[1
,2
]}

Battisti, Mario ^{[3
]}

De Martino, Selene ^{[3
]}

Nocerino, Valeria ^{[1
,4
]}

Dardano, Principia ^{[1
]}

De Stefano, Luca ^{[1
]}

Cangiano, Giancarlo ^{[5
]}

机构：

[1] Inst Appl Sci & Intelligent Syst ISASI, Natl Res Council, Via P Castellino 111, I-80131 Naples, Italy

[2] Univ Naples Federico II, DIETI, Via Claudio 21, I-80125 Naples, Italy

[3] Mat Srl, Corso Nicolangelo Protopisani 50, I-80146 Naples, Italy

[4] Univ Napoli Parthenope, Ctr Direzionale Napoli, Dept Engn, Isola C4, I-80143 Naples, Italy

[5] Co Di Me Srl, Corso Novara 10, I-80142 Naples, Italy

来源：

ADVANCED MATERIALS TECHNOLOGIES | 2023年 / 8卷 / 14期

关键词：

gold nanoparticles; hollow microneedles; hybrid devices; ISF extraction; plasmonic sensors; SKIN MODEL; DRUG; INSERTION; FABRICATION; ELECTRODES; MECHANISM; DELIVERY; SYSTEMS; DESIGN; ARRAY;

D O I：

10.1002/admt.202300037

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Interstitial fluid (ISF) extraction and analysis are challenges that can be tackled by Hollow MicroNeedles (HMNs) technology, overcoming most of the difficulties associated with in situ detection. Herein, a plasmonic transducer, composed of gold nanoparticles embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels, is integrated in the inner cavity of HMNs to detect biomarkers from the ISF-based point-of-care. The wearable HMN-based patch is used for minimally invasive pierce of the skin. The large swelling capability of the plasmonic transducer allows the uptake of ISF by capillarity. Biotin, as a small model molecule, is efficiently collected in the inner cavity of HMN and its high specificity with the streptavidin is exploited as a validation of the plasmonic nanocomposite functionality embedded within. The recognition of biotin is achieved in dual-optical mode: the localized surface plasmon resonance (label-free) and the metal-enhanced fluorescence (label-based). Overall, the proposed HMN-based patch for target sensing in ISF can represent a novel point-of-use device for the detection of biomarkers as an alternative to conventional hospital or lab settings to help faster medical decision-making.

引用

页数：15

共 69 条

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