Chemical profiling of Ziziphi spinosae semen using on-line comprehensive two-dimensional liquid chromatography-mass spectrometry based on a novel phthalic anhydride bonded stationary phase

被引：4

作者：

Zhao, Xinwei ^{[1
,2
]}

Yu, Dongping ^{[3
]}

Zhou, Weijia ^{[4
]}

Yu, Long ^{[1
]}

Zhou, Han ^{[1
]}

Liu, Yanfang ^{[1
,3
]}

Guo, Zhimou ^{[1
]}

Shen, Aijing ^{[1
]}

Han, Ziwei ^{[3
]}

Wang, Chaoran ^{[1
]}

Wang, Jixia ^{[1
,3
]}

Liang, Xinmiao ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Zhongshan Rd 457, Dalian 116023, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Ganjiang Chinese Med Innovat Ctr, Jiangxi Prov Key Lab Pharmacodynam Mat Basis Tradi, Nanchang, Peoples R China

[4] Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2023年 / 46卷 / 10期

关键词：

mass spectrometry; phthalic anhydride bonded stationary phase; reversed-phase liquid chromatography; two-dimensional liquid chromatography; Ziziphi spinosae semen; CYCLOPEPTIDE ALKALOIDS; PEAK-CAPACITY; SAPONINS; SEEDS; IDENTIFICATION; ELUCIDATION; SEPARATION; COLUMN;

D O I：

10.1002/jssc.202200704

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ziziphi spinosae semen has been widely used to treat insomnia and anxiety. To profile its chemical components, an online comprehensive two-dimensional liquid chromatography-mass spectrometry was developed. In this two-dimensional liquid chromatography system, a novel phthalic anhydride-bonded stationary phase column was combined with a C18 column. As a result, this new stationary phase exhibited remarkable differences in separation selectivity from C18, achieving a good orthogonality of 83.3%. Moreover, this new stationary phase with weaker hydrophobicity than C18 realized solvent compatibility in the online configuration. Coupled with tandem MS, 154 compounds were identified, including 51 unreported compounds. Compared with one-dimensional liquid chromatography-mass spectrometry, this online two-dimensional liquid chromatography-mass spectrometry system exhibited a much higher resolving power in isomer separation. This work provided an effective separation and characterization method for the material basis of Ziziphi spinosae semen. This strategy provides ideas for the material basis research of other traditional Chinese medicines.

引用

页数：14

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