Effect of alkaline pH-shifting process on extraction rate, structural, and functional properties of black soldier fly (Hermetia illucens) larvae protein

被引：11
|
作者：
Pan, Jiayin [1 ,2 ]
Xu, Haining [1 ,2 ]
Dabbour, Mokhtar [3 ]
Mintah, Benjamin Kumah [4 ]
Chen, Wen [1 ,2 ]
Yang, Fan [1 ,2 ]
Zhang, Zhaoli [5 ]
Cheng, Yu [1 ,2 ]
Dai, Chunhua [1 ,2 ]
He, Ronghai [1 ,2 ]
Ma, Haile [1 ,2 ]
机构：
[1] Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China
[2] Jiangsu Univ, Inst Food Phys Proc, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China
[3] Benha Univ, Dept Agr & Biosyst Engn, POB 13736, Moshtohor, Qaluobia, Egypt
[4] CSIR Food Res Inst, POB M20, Accra, Ghana
[5] Yangzhou Univ, Sch Food Sci & Engn, 196 Huayang West Rd, Yangzhou 225127, Jiangsu, Peoples R China
来源：
LWT-FOOD SCIENCE AND TECHNOLOGY | 2023年 / 185卷
基金：
中国国家自然科学基金;
关键词：
Black soldier fly larvae; Insect protein; pH-shifting extraction; Structural properties; Functional properties; ISOLATE;
D O I：
10.1016/j.lwt.2023.115180
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The effect of pH-shifting (10.5, 11.0, 11.5, 12.0, and 12.5) on the extraction rate, structural, and functional properties of black soldier fly larvae protein (BSFLP) was studied. The results showed that alkaline pH-shifting increased the extraction rate of BSFLP by 135-1080% (relative to the control). Fourier transform infrared and circular dichroism spectroscopy showed structural changes in BSFLP. The protein extracted under extreme alkaline pH (12.0, 12.5) was partially unfolded, leading to a substantial reduction in the disulfide bonds, hydrogen bonds, & alpha;-helix, and particle size; whereas a notable increase in the absolute zeta potential, surface hydrophobicity, and protein dispersion were realized. The solubility, foaming, and emulsifying properties of BSFLP were significantly improved (especially at pH 12.5). The outcome of the study provides a better understanding of the changes in structural traits and functionality of BSFLP - important for its utilization in the industry.
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页数：9
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