Size-selective downstream processing of virus particles and non-enveloped virus-like particles

被引：13

作者：

Hillebrandt, Nils ^{[1
]}

Hubbuch, Juergen ^{[1
]}

机构：

[1] Karlsruhe Inst Technol KIT, Inst Engn Life Sci, Sec Biomol Separat Engn 4, Karlsruhe, Germany

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2023年 / 11卷

关键词：

virus particles; virus-like particles; vaccines; downstream processing; purification; size-selective; INFLUENZA-A; CHROMATOGRAPHIC PURIFICATION; PROTEIN; VACCINES; PRECIPITATION; STABILITY; DELIVERY; PLATFORM; VECTORS; CAPTURE;

D O I：

10.3389/fbioe.2023.1192050

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Non-enveloped virus-like particles (VLPs) are versatile protein nanoparticles with great potential for biopharmaceutical applications. However, conventional protein downstream processing (DSP) and platform processes are often not easily applicable due to the large size of VLPs and virus particles (VPs) in general. The application of size-selective separation techniques offers to exploit the size difference between VPs and common host-cell impurities. Moreover, size-selective separation techniques offer the potential for wide applicability across different VPs. In this work, basic principles and applications of size-selective separation techniques are reviewed to highlight their potential in DSP of VPs. Finally, specific DSP steps for non-enveloped VLPs and their subunits are reviewed as well as the potential applications and benefits of size-selective separation techniques are shown.

引用

页数：8

共 87 条

[1]

Wingfield P, 2001, Curr Protoc Protein Sci, VAppendix 3, p3F, DOI [10.1002/0471140864.psa03fs84, 10.1002/0471140864.psa03fs13]

[2] Conformational stability and disassembly of Norwalk virus-like particles - Effect of pH and temperature [J].