Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

被引：5

作者：

Zhang, Teliang ^{[1
]}

Wang, Xueliang ^{[1
]}

Zeng, Youjun ^{[2
]}

Huang, Songfeng ^{[1
]}

Dai, Xiaoqi ^{[1
]}

Kong, Weifu ^{[1
]}

Liu, Qian ^{[1
]}

Chen, Jiajie ^{[1
]}

Qu, Junle ^{[1
]}

Shao, Yonghong ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen 518060, Peoples R China

[2] Guangdong Univ Technol, Coll Phys & Optoelect Engn, Guangzhou 510006, Peoples R China

来源：

BIOSENSORS-BASEL | 2023年 / 13卷 / 02期

基金：

中国国家自然科学基金;

关键词：

total internal reflection; surface plasmon resonance; surface plasmon resonance microscopy; biomolecular interaction;

D O I：

10.3390/bios13020261

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.

引用

页数：10

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