Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

被引:5
|
作者
Zhang, Teliang [1 ]
Wang, Xueliang [1 ]
Zeng, Youjun [2 ]
Huang, Songfeng [1 ]
Dai, Xiaoqi [1 ]
Kong, Weifu [1 ]
Liu, Qian [1 ]
Chen, Jiajie [1 ]
Qu, Junle [1 ]
Shao, Yonghong [1 ]
机构
[1] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen 518060, Peoples R China
[2] Guangdong Univ Technol, Coll Phys & Optoelect Engn, Guangzhou 510006, Peoples R China
来源
BIOSENSORS-BASEL | 2023年 / 13卷 / 02期
基金
中国国家自然科学基金;
关键词
total internal reflection; surface plasmon resonance; surface plasmon resonance microscopy; biomolecular interaction;
D O I
10.3390/bios13020261
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.
引用
收藏
页数:10
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