Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

被引：5

作者：

Zhang, Teliang ^{[1
]}

Wang, Xueliang ^{[1
]}

Zeng, Youjun ^{[2
]}

Huang, Songfeng ^{[1
]}

Dai, Xiaoqi ^{[1
]}

Kong, Weifu ^{[1
]}

Liu, Qian ^{[1
]}

Chen, Jiajie ^{[1
]}

Qu, Junle ^{[1
]}

Shao, Yonghong ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen 518060, Peoples R China

[2] Guangdong Univ Technol, Coll Phys & Optoelect Engn, Guangzhou 510006, Peoples R China

来源：

BIOSENSORS-BASEL | 2023年 / 13卷 / 02期

基金：

中国国家自然科学基金;

关键词：

total internal reflection; surface plasmon resonance; surface plasmon resonance microscopy; biomolecular interaction;

D O I：

10.3390/bios13020261

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.

引用

页数：10

共 20 条

[1] Advances in Surface Plasmon Resonance Imaging and Microscopy and Their Biological Applications [J].

Bockova, Marketa ;

Slaby, Jiri ;

Springer, Tomas ;

Homola, Jiri .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 12, 2019, 12 :151-176

[2]

Cai H.Y., 2014, CHIN OPT, V7, P691

[3] Optothermophoretic flipping method for biomolecule interaction enhancement [J].

Chen, Jiajie ;

Zeng, Youjun ;

Zhou, Jie ;

Wang, Xueliang ;

Jia, Boliang ;

Miyan, Ruibiao ;

Zhang, Teliang ;

Sang, Wei ;

Wang, Ying ;

Qiu, Haixia ;

Qu, Junle ;

Ho, Ho-Pui ;

Gao, Bruce Zhi ;

Shao, Yonghong ;

Gu, Ying .

BIOSENSORS & BIOELECTRONICS, 2022, 204

[4] Microscopy image resolution improvement by deconvolution of complex fields [J].

Cotte, Yann ;

Toy, M. Fatih ;

Pavillon, Nicolas ;

Depeursinge, Christian .

OPTICS EXPRESS, 2010, 18 (19) :19462-19478

[5] Single-Nanoparticle Near-Infrared Surface Plasmon Resonance Microscopy for Real-Time Measurements of DNA Hybridization Adsorption [J].

Halpern, Aaron R. ;

Wood, Jennifer B. ;

Wang, Yong ;

Corn, Robert M. .

ACS NANO, 2014, 8 (01) :1022-1030

[6] Point Spread Function of Objective-Based Surface Plasmon Resonance Microscopy [J].

Jiang, Yingyan ;

Wang, Wei .

ANALYTICAL CHEMISTRY, 2018, 90 (15) :9650-9656

[7] OPTICAL CONSTANTS OF NOBLE METALS [J].

JOHNSON, PB ;

CHRISTY, RW .

PHYSICAL REVIEW B, 1972, 6 (12) :4370-4379

[8]

Liu J.Z., 2014, THESIS NANKAI U TIAN

[9] Antimicrobial Susceptibility Test with Plasmonic Imaging and Tracking of Single Bacterial Motions on Nanometer Scale [J].

Syal, Karan ;

Iriya, Rafael ;

Yang, Yunze ;

Yu, Hui ;

Wang, Shaopeng ;

Haydel, Shelley E. ;

Chen, Hong-Yuan ;

Tao, Nongjian .

ACS NANO, 2016, 10 (01) :845-852

[10] EFFECTS OF NOISE, TIME-DOMAIN DAMPING, ZERO-FILLING AND THE FFT ALGORITHM ON THE EXACT INTERPOLATION OF FAST FOURIER-TRANSFORM SPECTRA [J].

VERDUN, FR ;

GIANCASPRO, C ;

MARSHALL, AG .

APPLIED SPECTROSCOPY, 1988, 42 (05) :715-721

← 1 2 →