Synthesis of 5-[4′-(1′′,3′′,7′′-Trimethyixanth-2′′-yl)phenyl]-10,15,20-tris-(N-methylpyridinium-3′-yl)porphyrin Triiodide and Features of Its Reaction with Poly[d(AT)2]

被引:3
|
作者
Syrbu, S. A. [1 ,2 ]
Kiselev, A. N. [1 ,2 ]
Lebedev, M. A. [1 ,2 ]
Yurina, E. S. [1 ]
Lebedeva, N. Sh. [1 ]
机构
[1] Russian Acad Sci, GA Krestov Inst Solut Chem, Ivanovo 153045, Russia
[2] Ivanovo State Univ Chem & Technol, Ivanovo 153000, Russia
基金
俄罗斯科学基金会;
关键词
porphyrins; synthesis; oligonucleotides; complexation; photoinactivation; BINDING; DNA; PORPHYRINS; FUNCTIONALIZATION;
D O I
10.1134/S1070363223150197
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Directed synthesis of a new cationic monoheteryl-substituted porphyrin, 5-[4 '-(1 '',3 '',7 ''-trimethyixanth2 ''-yl]-10,15,20-tris-(N-methylpyridinium-3 '-yl) porphyrin triiodide was carried out. This porphyrin is in a partially associated state in Tris-buffer solutions at pH = 7.4, and has a long fluorescence lifetime (similar to 9 ns). It has been found that the synthetic nucleic acid binds the synthesized porphyrin in the groove with an affinity constant of 2.3x10(7). It has been shown that the porphyrin binding by poly[d(AT)(2)] leads to a decrease in the particle size. Photoirradiation of the porphyrin complex with poly[d(AT)(2)] by visible light leads to the destruction of the nucleic acid and enhances the porphyrin photolysis.
引用
收藏
页码:S562 / S571
页数:10
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