Phenolic glycosides and indole alkaloids isolated from the roots and rhizomes of Clematis chinensis and their anti-inflammatory activity

被引：0

作者：

Jiang, Hai -Bing ^{[1
]}

Shao, Si -Yuan ^{[1
]}

Wei, Ya-Zi ^{[1
]}

Lin, Ming-Bao ^{[1
]}

Li, Shuai ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing 100050, Peoples R China

来源：

PHYTOCHEMISTRY | 2023年 / 215卷

关键词：

Clematis chinensis; Ranunculaceae; Indole alkaloids; Phenolic glycosides; Anti-inflammatory activity; TNF-alpha and IL-6 inhibitory activities; NF -KB signaling pathway; TRITERPENOID SAPONINS; CONSTITUENTS; ALLOPYRANOSIDES; BARK;

D O I：

10.1016/j.phytochem.2023.113832

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six undescribed compounds, including three phenolic glycosides (1-3) and three indole alkaloids (4-6), together with ten known alkaloids (7-16) and three known phenolic glycosides (17-19), were isolated from 70% EtOH aqueous extracts of the roots and rhizomes of Clematis chinensis Osbeck. The structures were elucidated by NMR, HRESIMS and X-ray diffraction spectroscopies. The anti-inflammatory activity of these compounds was evaluated, and twelve compounds showed significant inhibitory activity against TNF-alpha with an inhibition ratio from 47.87% to 94.70% at a dose of 10 mu M. Compound 7 exhibited significant inhibitory activity against TNF-alpha and IL-6 with IC50 values of 3.99 mu M and 2.24 mu M, respectively. Compound 8 displayed potent anti-inflammatory activity against mouse ear edema induced by croton oil. A mechanistic study suggested that compounds 7 and 8 decreased the activation of the NF-kappa B signaling pathway to reduce the secretion of inflammatory factors in LPS-induced RAW 264.7 cells.

引用

页数：10

共 42 条

[1]

[Anonymous], 2004, J. Chin. Pharm. Sci., DOI [10.1147/sj.421.0045, DOI 10.1147/SJ.421.0045]

[2] Monoindole alkaloids from a marine sponge Spongosorites sp. [J].